Immunologic and molecular identification of Babesia bovis and Babesia bigemina in free-ranging white-tailed deer in northern Mexico
Immunologic and molecular identification of Babesia bovis and Babesia bigemina in free-ranging white-tailed deer in northern Mexico
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DOI：
10.7589/0090-3558-43.3.504
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发表时间：
2007-07-01
影响因子：
1.3
通讯作者：
George, John E.
中科院分区：
文献类型：
--
作者：
Cantu, Antonio;Ortega-S, J. Alfonso;George, John E.
白尾鹿（Odocoileus virginianus）作为牛蜱Rhipicephalus microplus和Rhipicephalus annulatus宿主的适宜性已有很好的记录。这些蜱具有广泛的宿主范围，并且都传播引起牛巴贝斯虫病的病原体牛巴贝斯虫和二联巴贝斯虫。虽然这种疾病及其媒介已经在美国和墨西哥北方的一些州被根除，但它在墨西哥其他州仍然是一个问题。目前还不知道野生鹿科动物如白尾鹿是否可以作为牛巴贝虫病的宿主。本研究的目的是确定B. bovis和B.在墨西哥的新莱昂州和塔毛利帕斯州的白尾鹿中出现了双胎或针对它们的抗体。采用巢式聚合酶链反应（nested PCR）和间接免疫荧光抗体试验（IFAT）对2个牧场的20份白尾鹿血液样品进行了B的检测。bovis和B.二联。11份样本对B呈阳性。二联胺和四个B。结果表明，扩增产物序列与GenBank中报道的B基因序列完全一致。bovis（Rap 1）和B.二联。IFA试验结果显示血清样品中存在特异性抗体。这是首次报道B的存在。bovis和B.使用这些技术在白尾鹿的bigemina，并强调了鹿科动物作为牛巴贝虫病可能的水库的重要性。
The suitability of white-tailed deer (Odocoileus virginianus) as hosts for the cattle ticks Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus, has been well documented. These ticks have a wide host range, and both transmit Babesia bovis and Babesia bigemina, the agents responsible for bovine babesiosis. Although this disease and its vectors have been eradicated from the United States and some states in northern Mexico, it still is a problem in other Mexican states. It is not known if wild cervids like white-tailed deer can act as reservoirs for bovine babesiosis. The purpose of this study was to determine if B. bovis and B. bigemina or antibodies against them occur in white-tailed deer in the states of Nuevo Leon and Tamaulipas, Mexico. Twenty blood samples from white-tailed deer from two ranches were collected and tested with a nested polymerase chain reaction (nested PCR) and indirect immunofluorescence antibody test (IFAT) for B. bovis and B. bigemina. Eleven samples were positive for B. bigemina and four for B. bovis by nested PCR; amplicon sequences were identical to those reported in GenBank for B. bovis (Rap 1) and B. bigemina. Results of the IFA test showed the presence of specific antibodies in serum samples. This is the first report of the presence of B. bovis and B. bigemina in white-tailed deer using these techniques and underscores the importance of cervids as possible reservoirs for bovine babesiosis.