ALTERED ION-TRANSPORT IN AORTIC SMOOTH-MUSCLE DURING DEOXYCORTICOSTERONE ACETATE HYPERTENSION IN RAT
ALTERED ION-TRANSPORT IN AORTIC SMOOTH-MUSCLE DURING DEOXYCORTICOSTERONE ACETATE HYPERTENSION IN RAT
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DOI：
10.1161/01.res.37.3.333
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发表时间：
1975-01-01
影响因子：
20.1
通讯作者：
HART, RG
中科院分区：
文献类型：
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作者：
JONES, AW;HART, RG
研究了大鼠醋酸脱氧皮质酮（DOCA）高血压发生过程中主动脉水电解质分布及离子转换的变化。在高血压前期，DOCA加生理盐水治疗与42K周转率（0.0142 +/- 0.0005 vs 0.0102 +/- 0.0003 min-1）、细胞水分（0.89 +/- 0.03 vs 0.76 +/- 0.02 kg/kg干重）和体重/长度比的增加相关。这些参数在高血压的发展过程中进一步增加。总钾、钙、镁含量以及胞外空间校正后的Na和C1含量也显著增加。DOCA高血压大鼠36Cl的周转量增加（0.230 +/- 0.006比0.136 +/- 0.004 min-1），慢交换Cl含量增加。细胞外Ca的去除大大增加了42K的稳态周转。对于对照大鼠，0.1 mM的Ca浓度使42K周转率降低到Ca无值的50%以下（0.063 +/- 0.004 min-1），而DOCA高血压大鼠仅降低10%。在Ca浓度最高（5 mM）时，doca治疗大鼠的42K周转更大，高血压患者的最大外排量为67%，约为对照组外排量的两倍。由此可见，血管平滑肌离子转运的显著改变发生在DOCA加生理盐水治疗高血压发病前和发病期间。这种变化可能是由于钙稳定膜的能力降低所致。我们认为这种改变有助于血管反应性的改变和高血压相关的肥厚。
Change in aortic water and electrolyte distribution and in ion turnover were studied during the development of deoxycorticosterone acetate (DOCA) hypertension in the rat. Treatment with DOCA plus saline during the prehypertensive phase was associated with increases in 42K turnover (0.0142 +/- 0.0005 vs. 0.0102 +/- 0.0003 min-1), cell water (0.89 +/- 0.03 vs 0.76 +/- 0.02 kg/kg dry weight), and the ratio of weight to length. These parameters were further increased during the development of hypertension. Significant increases were also observed in total K, Ca, and Mg contents and in Na and C1 contents corrected for the extracellular space. The turnover of 36Cl was increased (0.230 +/- 0.006 vs. 0.136 +/- 0.004 min-1) in DOCA hypertensive rats as was the content of slowly exchanging Cl. Removal of extracellular Ca greatly increased the steady-state turnover of 42K. For control rats, a Ca concentration of 0.1 mM reduced the rate of 42K turnover to less than 50% of the Ca-free value (0.063 +/- 0.004 min-1), whereas DOCA hypertensive rats exhibited only a 10% reduction. At the highest Ca concentration, 5 mM, the 42K turnover was greater in DOCA-treated rats with the hypertensives operating at 67% of maximum efflux or about twice the efflux in controls. It is concluded that significant alterations in ion transport by vascular smooth muscle occur before and during the development of hypertension induced by treatment with DOCA plus saline. Such changes may result from a reduced ability of Ca to stabilize the membrane. It is proposed that such alterations contribute to the changes in vascular reactivity and the hypertrophy associated with hypertension.