Discriminating between Ixodes ticks by means of mitochondrial DNA sequences
Discriminating between Ixodes ticks by means of mitochondrial DNA sequences
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DOI：
10.1006/mpev.1995.1033
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发表时间：
1995-12-01
影响因子：
4.1
通讯作者：
Kocher, TD
中科院分区：
文献类型：
--
作者：
Caporale, DA;Rich, SM;Kocher, TD
硬蜱属蜱最近受到重视，因为它们经常作为重要人畜共患病的载体，包括莱姆病和巴贝西虫病。用于识别它们的形态特征通常是不明确的，并且仅在特定的发育阶段有用。在这里，我们报告了九种不同的硬蜱和另一个属的外类群 Dermacentor 的线粒体编码 16S rRNA 基因的 DNA 序列。这些序列很容易区分这些蜱虫。来自美国东北部和中西部上部的 I. dammini 样本与东南部的 I. scapularis 样本的核苷酸差异约为 2%。这种差异大约是蓖麻属物种其他成员之间差异的一半，但超出了种内变异的典型水平。蓖麻复合体内存在两个主要分支。其中包括 I. Cookei、I. hexagonus 和 I. angustus。其他包括 I. persulcatus、I. pacificus、I. muris、I. ricinus、LI scapularis 和 I. dammini。我们得出的结论是，mtDNA 序列有助于阐明这些人类疾病重要载体的系统学。 (C) 1995 学术出版社
Ticks of the genus Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis, The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular stage of development. Here we report the DNA sequence of the mitochondrially encoded 16S rRNA gene of nine different Ixodes ticks and an outgroup from another genus, Dermacentor. The sequences readily discriminate between these ticks. Samples of I. dammini from the northeastern and upper midwestern United States differ from southeastern I. scapularis at about 2% of the nucleotides. This difference is about half that separating other members of the I. ricinus group of species, but exceeds typical levels of intraspecific variation. Two major clades exist within the I. ricinus complex. One includes I. cookei, I. hexagonus, and I. angustus. The other includes I. persulcatus, I. pacificus, I. muris, I. ricinus, LI scapularis, and I. dammini. We conclude that mtDNA sequences are useful for unravelling the systematics of these important vectors of human disease. (C) 1995 Academic Press, Inc.