Bio-enhanced activated carbon filter with immobilized microorganisms for removing organic pollutants in the Songhua River
Bio-enhanced activated carbon filter with immobilized microorganisms for removing organic pollutants in the Songhua River
复制标题
DOI：
10.2166/wst.2010.666
复制
发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Wang, Guang-Zhi
中科院分区：
文献类型：
--
作者：
Gao, Yu-Nan;Li, Wei-Guang;Wang, Guang-Zhi
从松花江水体中分离到5种优势微生物，包括4种假单胞菌和1种芽孢杆菌。研究了固定化生物强化活性炭滤池（BEAC）对有机污染物的去除潜力和群落组成，并与自然形成的生物活性炭滤池（BAC）进行了比较。采用松花江水作为原水。中试结果表明，运行45 d后，BEAC滤池生物量先增加后稳定，平均值为192 nmolPO（4）/g炭。相应的生物活性达到1，368 ng ATP/g碳。气相色谱-质谱联用（GC-MS）结果表明，BEAC滤料对有毒有机物的降解效果优于BAC滤料，尤其是对卤代烃和多环芳烃的降解效果更好。聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）分析表明，5个优势微生物的生态系统并没有改变，即使在180 d的操作BEAC过滤器。其中枯草芽孢杆菌和巴利阿里假单胞菌具有较高的生物活性，对碳源表面的适应性强于其他3种优势菌。扫描电子显微镜（SEM）照片显示BEAC过滤器上生长了大量微生物。采用Deltatox生物分析仪对优势菌进行毒性试验，结果表明优势菌在饮用水处理工艺中应用是安全的。
Five dominant microorganisms including four kinds of Pseudomonas and one kind of Bacillus were isolated from the Songhua River. The organic pollutants removal potential and community composition of these five dominant microorganisms immobilized on activated carbon filter, which is called the bio-enhanced activated carbon filter (BEAC), were investigated to compare with the naturally formed biological activated carbon (BAC) filter. Songhua River was used as the raw water. The pilot scale test results showed the biomass in the BEAC filter increased initially and then stabilized after 45 d of operation with an average value of 192 nmolPO(4)/g carbon. The corresponding biological activity reached 1,368 ng ATP/g carbon. The gas chromatography-mass spectrometry (GC-MS) results showed that the BEAC filter degraded the toxic organic substances more effectively than the BAC filter, especially for halogenated hydrocarbons and PAHs (polycyclic aromatic hydrocarbons). Polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis revealed the eco-system of five dominant microorganisms did not change in the BEAC filter even on 180 d of operation. Two of the five dominant microorganisms, Bacillus subtilis and Pseudomonas balearica, had high biological activity and were more adaptable to the surface of the carbon media than the other three dominant microorganisms. The scanning electron microscope (SEM) photograph showed a large quantity of microorganisms developed on the BEAC filter. The toxicity test using Deltatox Bioassay Technology Analyzer indicated that the dominant microorganisms were safe to be applied in drinking water treatment process.