Recombination between two identical sequences within the same retroviral RNA molecule
Recombination between two identical sequences within the same retroviral RNA molecule
复制标题
DOI：
10.1128/jvi.73.7.5912-5917.1999
复制
发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Sapp, CM
中科院分区：
文献类型：
--
作者：
Zhang, JY;Sapp, CM
As a consequence of being diploid viruses, members of the Retroviridae have a high recombination rate. To measure recombination between two identical sequences within the same RNA molecule per round of retroviral replication cycle, a murine leukemia virus based vector (JZ442 + 3' Hyg) has been constructed. It carries a drug resistance gene, hyg, and a 290-bp repeat sequence of the 3' hyg gene inserted into the 3' untranslated region of the green fluorescent protein gene (gfp). Under fluorescence microscopy, Hyg(r) cells containing the recombinant proviruses were clear, while a green color was observed in the drug-resistant cells carrying the parental proviruses, The rate of recombination was determined by the ratio of the number of clear colonies to the total number of Hyg(r) colonies (green and clear colonies). The rate of recombination was found to be 62% by this method. The intermolecular recombination rate between an infectious virus bearing two copies of the 290-bp segment and a noninfectious chimeric RNA virus containing only a single copy of this sequence was also measured.
