DYE UPTAKE METHODS FOR ASSESSING VIRAL CYTOPATHOGENICITY AND THEIR APPLICATION TO INTERFERON ASSAYS
DYE UPTAKE METHODS FOR ASSESSING VIRAL CYTOPATHOGENICITY AND THEIR APPLICATION TO INTERFERON ASSAYS
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DOI：
10.1099/0022-1317-5-3-419
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发表时间：
1969-01-01
影响因子：
3.8
通讯作者：
FINTER, NB
中科院分区：
文献类型：
--
作者：
FINTER, NB
当中性红加入到组织培养的液体培养基中时，健康细胞比受损细胞吸收更多的染料。这形成了用于评估不同培养物中细胞损伤的相对程度的方法的基础，通过检查染色的细胞片直接确定结合染料的量，或通过比色法估计提取的染料间接确定结合染料的量。该方法可用于测定杀细胞病毒的作用，也可用于干扰素的测定。本文提供了一种精确和可重复的方法，该方法对测定小鼠干扰素异常敏感，并且也已用于测定大鼠、鸡、兔和人干扰素。
When neutral red is added to the liquid medium in tissue cultures, more dye is taken up by healthy cells than by damaged cells. This forms the basis of a method for assessing the relative extent of cell damage in different cultures, the amounts of bound dye being determined directly by inspection of the stained cell sheets, or indirectly by estimating extracted dye colorimetrically. This procedure can be used to measure the effects of a cytocidal virus and can also be applied to the assay of interferons. Here it provides a precise and reproducible method which is unusually sensitive for the assay of mouse interferon, and has also been used to assay rat, chick, rabbit and human interferons.