RNA-catalyzed CoA, NAD, and FAD synthesis from phosphopantetheine, NMN, and FMN
RNA-catalyzed CoA, NAD, and FAD synthesis from phosphopantetheine, NMN, and FMN
复制标题
DOI：
10.1021/bi002061f
复制
发表时间：
2000-12-19
期刊：
影响因子：
2.9
通讯作者：
Yarus, M
中科院分区：
文献类型：
--
作者：
Huang, FQ;Bugg, CW;Yarus, M
建立了一种新的体外筛选方法来分离具有辅酶合成活性的RNA序列。我们使用了大小不同的文库，其中包含四种不同长度（30N, 60N， 100N和140N）的随机核糖核苷酸序列，均为5'-ATP起始。CoES7 （30N）和CoES21 （60N）这两个rna能够分别催化4'-磷酸蜂氨酸、NMN和FMN的前体合成三种常见的辅酶：CoA、NAD和FAD。两种核酶的活性都需要二价锰。这些结果支持了这些辅酶在RNA世界中的可用性，并指出了许多辅酶复杂的二元结构的化学解释。
A novel in vitro selection method was developed to isolate RNA sequences with coenzyme-synthesizing activities. We used size-heterogeneous libraries containing randomized ribonucleotide sequences of four different lengths (30N, 60N, 100N, and 140N), all with 5'-ATP initiation. Two RNAs, CoES7 (30N) and CoES21 (60N), are able to catalyze the synthesis of three common coenzymes, CoA, NAD, and FAD, from their precursors, 4'-phosphopantetheine, NMN, and FMN, respectively. Both ribozymes require divalent manganese for activities. The results support the availability of these coenzymes in an RNA world, and point to a. chemical explanation for the complex bipartite structures of many coenzymes.