Toxicity of lindane induced by oxidative stress and intestinal damage in Caenorhabditis elegans
Toxicity of lindane induced by oxidative stress and intestinal damage in Caenorhabditis elegans
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氧化应激和肠道损伤引起的林丹对秀丽隐杆线虫的毒性
DOI：
10.1016/j.envpol.2020.114731
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发表时间：
2020
影响因子：
8.9
通讯作者：
Xiang Mingdeng
中科院分区：
文献类型：
--
作者：
Yu Yunjiang;Hua Xin;Chen Haibo;Wang Ya'e;Li Zongrui;Han Yajing;Xiang Mingdeng
林丹是一种亲脂性污染物，可能对生物体有毒。为探讨林丹的毒性作用及其机制，采用秀丽隐杆线虫（Cahabditis elegans）动物模型。elegans）暴露于环境相关浓度（0.01-100 ng/L）的林丹3d，测定其生理生化指标和分子生物学指标。亚急性暴露于10-100 ng/L的林丹，可对中华绒螯蟹的发育、繁殖和运动行为产生不良的生理影响。优雅暴露于1-100 ng/L的亚麻酸会增加尼罗红和蓝色食用染料的积累，这表明线虫肠道的高渗透性。接触林丹还显著影响了与肠道发育有关的基因的表达（例如，mtm-6和opt-2）。此外，活性氧物质的产生、脂褐素的积累和抗氧化基因（例如，sod-5和isp-1）在C. 10-100 ng/L的亚麻酸暴露于线虫体内，表明亚麻酸暴露诱导了氧化应激。Pearson相关分析表明，氧化应激和肠道损伤与亚麻酸的不良生理效应显著相关。因此，林奈福的不良反应可能是由肠道损伤和氧化应激引起的，而mtm-6、opt-2、sod-5、isp-1和mev-1可能在林奈福的毒性中起重要作用。(C)2020爱思唯尔有限公司保留所有权利。
Lindane, a lipophilic pollutant, may be toxic to organisms. To explore the toxic effects of lindane and the underlying mechanisms of this toxicity, the animal model Caenorhabditis elegans (C. elegans) was exposed to lindane for 3 d at environmentally relevant concentrations (0.01-100 ng/L) and the physiological, biochemical, and molecular indices were evaluated. Subacute exposure to 10-100 ng/L of lindane caused adverse physiological effects on the development, reproduction, and locomotion behaviors in C. elegans. Exposure to 1-100 ng/L of lindane increased the accumulation of Nile red and blue food dye, which suggested high permeability of the intestine in nematodes. Lindane exposure also significantly influenced the expression of genes related to intestinal development (e.g., mtm-6 and opt-2). Moreover, reactive oxygen species production, lipofuscin accumulation, and expression of oxidation resistance genes (e.g., sod-5 and isp-1) were significantly increased in C. elegans exposed to 10-100 ng/L of lindane, which indicated that lindane exposure induced oxidative stress. According to Pearson correlation analyses, oxidative stress and intestinal damage were significantly correlated with the adverse physiological effects of lindane. Therefore, the adverse effects of lindane may have been induced by intestinal damage and oxidative stress, and mtm-6, opt-2, sod-5, isp-1, and mev-1 might play important roles in the toxicity of lindane. (C) 2020 Elsevier Ltd. All rights reserved.