Production, characterization and evaluation of virulence of an adhesion defective mutant of Flavobacterium columnare produced by β-lactam selection
Production, characterization and evaluation of virulence of an adhesion defective mutant of Flavobacterium columnare produced by β-lactam selection
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DOI：
10.1111/j.1472-765x.2004.01641.x
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Klesius, PH
中科院分区：
文献类型：
--
作者：
Bader, JA;Shoemaker, CA;Klesius, PH
目的：对引起鱼类柱状菌病的柱状黄杆菌进行突变体的开发和鉴定。方法与结果：在富含氨苄西林（β -内酰胺）的改良Hsu-Shotts培养基上连续传代，获得了一个在菌落形态、全细胞蛋白、粘附力和毒力方面与亲本菌株不同的柱状芽孢杆菌突变体。该突变体在浸渍侵染期间的毒力与亲本不同，但在注射侵染或产生抗体时则不同。结论：柱状黄杆菌暴露于氨苄西林既产生对抗生素的耐药性，又产生缺乏或减少粘附特性和改变粘附鱼组织能力的突变体。研究的意义和影响：这是首次描述柱状梭菌黏附缺陷突变体以及黏附改变对柱状梭菌疾病的影响。该突变体具有相当大的潜力，可作为研究粘连在柱状体疾病中的作用的工具。
Aims: The aim of this study was to develop and evaluate mutants of Flavobacterium columnare, the causative agent of columnaris disease in fish.Methods and Results: Serial passage on ampicillin (beta-lactam) enriched modified Hsu-Shotts medium resulted in a F. columnare mutant that differed from the parent strain in colony morphology, whole cell proteins, adhesion and virulence. The mutant differed from its parent in virulence during immersion challenge, but not during injection challenge or generation of antibodies.Conclusion: Flavobacterium columnare exposure to ampicillin produces both resistance to that antibiotic and produces mutants that lack or have reduced adhesion characteristics and modified ability to adhere to fish tissue.Significance and Impact of the Study: This is the first description of an adhesion-defective mutant of F. columnare and the effects of altered adhesion on columnaris disease. This mutant has considerable potential as a tool to study the role of adhesion in columnaris disease.