Guanylate cyclase activity and cyclic nucleotide concentrations during liver regeneration after experimental injury.
Guanylate cyclase activity and cyclic nucleotide concentrations during liver regeneration after experimental injury.
复制标题
实验损伤后肝再生过程中鸟苷酸环化酶活性和环核苷酸浓度。
DOI：
10.1042/bj1640033
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
W. Reutter
中科院分区：
文献类型：
--
作者：
C. Goridis;J. Zwiller;W. Reutter
测定再生大鼠肝脏环核苷酸浓度和鸟苷酸环化酶活性。先前的研究表明，在部分肝切除的大鼠肝脏中，膜结合鸟苷酸环化酶的活性在早期复制阶段显著增加[Kimura & Murad (1975) Proc. Natl]。学会科学。U.S.A.72, 1965 - 1969;Goridis & Reutter（1975）《自然》（伦敦）257,698-700]。在肝部分切除后的同一时间内，戊巴比妥麻醉下杀死大鼠时发现组织中环GMP浓度升高，而不麻醉时则没有。在半乳糖胺治疗期间，将肝切除肝脏的结果与鸟苷酸环化酶活性和环核苷酸浓度的变化进行比较。这里，鸟苷酸环化酶活性和环GMP浓度的峰值出现在8h，即增生反应开始之前。在DNA合成增加时，这两项参数均正常。因此，鸟苷酸环化酶活性或环GMP浓度的变化与肝脏DNA合成的增加之间似乎没有一致的相关性。然而，在半乳糖胺处理的大鼠的肝脏中，发现环AMP浓度适度上升，这与DNA合成的时间一致。
Cyclic nucleotide concentrations and guanylate cyclase activity were measured in regenerating rat liver. Previous work has shown that in livers of partially hepatectomized rats the activity of a membrane-bound guanylate cyclase increases considerably during the early replicative phase [Kimura & Murad (1975) Proc. Natl. Acad. Sci. U.S.A.72, 1965-1969; Goridis & Reutter (1975) Nature (London) 257, 698-700]. Over the same time period after partial hepatectomy, increased tissue concentrations of cyclic GMP were found when the rats were killed under pentobarbital anaesthesia, but not when anaesthesia was omitted. The results obtained on hepatectomized livers were compared with the changes in guanylate cyclase activity and cyclic nucleotide concentrations during the response to galactosamine treatment. Here, a peak of guanylate cyclase activity and of cyclic GMP concentrations occurred at 8h, that is before the beginning of the proliferative response. Both parameters were normal at the time of increased DNA synthesis. There does not, therefore, seem to be a consistent correlation between changes in guanylate cyclase activity or concentrations of cyclic GMP and an increase in liver DNA synthesis. A modest rise in cyclic AMP concentrations was found, however, in livers of galactosamine-treated rats, which was coincident with the time of DNA synthesis.