Thermographic visualization of cell death in tobacco and Arabidopsis
Thermographic visualization of cell death in tobacco and Arabidopsis
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DOI：
10.1046/j.1365-3040.2001.00654.x
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发表时间：
2001-01-01
影响因子：
7.3
通讯作者：
Van Der Straeten, D
中科院分区：
文献类型：
--
作者：
Chaerle, L;De Boever, F;Van Der Straeten, D
在转细菌视蛋白的烟草植株中，热成像显示了待处理的细胞死亡，这些植物中的细胞死亡具有复杂的病斑表型，孤立的细胞死亡病变之前存在共局灶性热效应，这与以前在烟草花叶病毒(TMV)感染部位观察到的一样(Chaerle等人)。1999自然生物技术17,813-816)。然而，在大多数情况下，伴随着细胞死亡的是一个连贯的较高温度锋面，它始于叶基，并扩展到叶片上。与均匀的热锋相反，细胞死亡首先在静脉附近可见，随后随着细胞死亡沿静脉扩散，在静脉间组织上出现离散的斑点。由于受损细胞的晶片蒸发，可见细胞死亡的区域温度较低。与先前关于局域烟草-烟草花叶病毒相互作用的观察类似(Chaerle等人。1999年)，热像和连续气体交换测量的动力学表明，在组织崩溃之前，气孔关闭，局部的自发细胞死亡也可以在症状前在拟南芥lsd2突变体中可视化。
Pending cell death was visualized by thermographic imaging in bacterio-opsin transgenic tobacco plants, Cell death in these plants was characterized by a complex lesion phenotype, Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999 Nature Biotechnology 17, 813-816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of wafer evaporation from damaged cells. In analogy with previous observations on the-localized tobacco-TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse, Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant.