Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis
Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis
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DOI：
10.1111/jam.13011
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发表时间：
2016-02-01
影响因子：
4
通讯作者：
Son, A.
中科院分区：
文献类型：
--
作者：
Lim, H. J.;Lee, E. -H.;Son, A.
目的介绍一种用于快速单步提取细菌DNA的便携式裂解装置。方法和结果我们的便携式裂解装置采用了一种新颖的设计，由环形压电元件和穿孔隔膜组成。我们的便携式裂解装置以枯草芽孢杆菌为目标菌，能够在30s内实现高达66%的标准化裂解率。这与微探头超声的效果相当，几乎是传统头部敲打的7倍。添加玻璃微珠的效果是可以预见的。然而，加入十二烷基硫酸钠（SDS）的结果与直觉相反，因为从0.05浓度进一步增加到1%浓度会降低裂解性能。便携式裂解装置的功率效率至少是微探头超声的15-5倍。结论sour便携式裂解仪能够快速提取细菌DNA，且比探针超声更节能。添加玻璃微珠或SDS浓度（可达05%）可改善其性能。研究的意义和影响便携式裂解装置提供了一种独立、快速、低成本和节能的方法，可以在环境、生物医学和其他应用微生物学领域的各种生物测定之前获得基因组成分。
AimsTo demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction.Methods and ResultsOur portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 05 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 15-5 times more power efficient than microprobe ultrasonication.ConclusionsOur portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 05%) improves its performance.Significance and Impact of the StudyThe portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.