IDENTIFICATION OF ADENOSINE TRIPHOSPHATE IN HUMAN PLASMA AND CONCENTRATION IN VENOUS EFFLUENT OF FOREARM MUSCLES BEFORE, DURING AND AFTER SUSTAINED CONTRACTIONS
IDENTIFICATION OF ADENOSINE TRIPHOSPHATE IN HUMAN PLASMA AND CONCENTRATION IN VENOUS EFFLUENT OF FOREARM MUSCLES BEFORE, DURING AND AFTER SUSTAINED CONTRACTIONS
复制标题
DOI：
10.1113/jphysiol.1969.sp008917
复制
发表时间：
1969-01-01
影响因子：
5.5
通讯作者：
LIND, AR
中科院分区：
文献类型：
--
作者：
FORRESTER, T;LIND, AR
1. 当稀释的人血浆通过青蛙心脏灌注时，心跳明显增强，其作用与心脏上低浓度的三磷酸腺苷（ATP）非常相似。结果表明，血浆中引起心脏刺激的物质是ATP。使用了以下测试：(a)稀释的等离子体从萤火虫灯提取物中发射的光具有ATP溶液产生的光信号的特征；(b)血浆溶液与apyrase（将ATP转化为一磷酸腺苷（AMP））一起孵育，消除了对青蛙心脏的刺激作用和对萤火虫提取物的发光作用；AMP不会刺激心脏或使萤火虫提取物发出光；(c)血浆中的刺激物质通过Sephadex G‐25柱以与ATP相同的模式洗脱；(d)同时测定蛙心和萤火虫提取物血浆溶液的定量结果与ATP.3溶液的定量结果相同。静息组静脉血血浆ATP含量为0.19 ~ 0.95 μg/ml。(平均0.63 μg/ml, sd±0·25);检测到的多达一半的ATP可归因于血小板损伤。4名受试者静息时同时采集的动脉和静脉血样平均浓度为0.19 μg/ml。（0.07 ~ 0.26 μg/ml）和0.70 μg/ml。（0.57 ~ 0.84 μg/ml）。测定前臂肌肉运动后静脉流出液中ATP的浓度。在运动过程中，静脉血药浓度持续高于静息值，而有一名受试者在运动过程中动脉血药浓度几乎没有变化。由此得出结论，ATP是在血液通过肌床的过程中加入到血液中的。讨论了ATP的起源，包括红细胞、血小板和活跃的骨骼肌。
1. When diluted human plasma is perfused through a frog heart, a marked augmentation of the heartbeat is produced which is very similar in action to that of low concentrations of adenosine triphosphate (ATP) on the heart.2. It was established that the substance in the plasma responsible for the heart stimulation was ATP. The following tests were used: (a) the diluted plasma emitted light from firefly lantern extract characteristic of the light signal produced by a solution of ATP; (b) the stimulatory effect on the frog heart and luminescent effect upon the firefly extract were abolished by incubation of the plasma solution with the enzyme apyrase, which converts ATP to adenosine monophosphate (AMP); AMP does not stimulate the heart or cause light to be emitted from firefly extract; (c) the stimulatory substance in the plasma was eluted through a column of Sephadex G‐25 in the same pattern as ATP; and (d) simultaneous assay of plasma solutions on frog heart and firefly extract produced the same quantitative result as that produced by a solution of ATP.3. The amount of ATP in plasma from the venous blood of resting subjects ranged from 0·19 to 0·95 μg/ml. (mean 0·63 μg/ml.,S.D.± 0·25); up to half of the ATP detected could be attributed to blood platelet damage. Simultaneous arterial and venous samples of blood from four subjects at rest had mean concentrations of 0·19 μg/ml. (0·07–0·26 μg/ml.) and 0·70 μg/ml. (0·57–0·84 μg/ml.) respectively.4. The concentration of ATP in the venous effluent from exercising forearm muscles was measured. The venous concentration consistently increased over the resting values in response to exercise while in one subject little change occurred in the arterial blood concentration during the exercise. It was concluded that the ATP was added to the blood in its passage through the muscle bed.5. The origin of the ATP, including erythrocytes, blood platelets and active skeletal muscle, is discussed.