BACTERIAL DRY-MATTER CONTENT AND BIOMASS ESTIMATIONS

BACTERIAL DRY-MATTER CONTENT AND BIOMASS ESTIMATIONS
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DOI:
10.1128/aem.48.4.755-757.1984
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发表时间:
1984-01-01
影响因子:
4.4
通讯作者:
DUNDAS, I
DUNDAS, I
中科院分区:
生物学2区
文献类型:
--
作者:
BRATBAK, G;DUNDAS, I

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对于细菌细胞来说,大约20%的干物质含量似乎是一个可接受的标准值。细菌的干物质含量可能是通常假设的两倍以上。低估计的主要原因似乎是在估计细胞湿重时没有对细胞间水进行适当的修正。用3个不同的细菌菌株,得到的细胞干物质含量在31-57%之间,这表明接受的标准值太低,离标准很远。若要将细菌生物量换算为生物量(C含量),建议以0.22g C cm-3作为换算因子。
Approximately 20% dry-matter content appears to be an accepted standard value for bacterial cells. The dry-matter content of bacteria may be more than twice as high as generally assumed. The main reason for the low estimates seems to be that proper corrections for intercellular water were not made when estimating the wet weight of the cells. Using 3 different bacterial strains, a dry-matter content of cells ranging from 31-57% was obtained, suggesting that the accepted standard value is much too low and that it is far from standard. To convert bacterial biovolume into biomass (C content), it is suggested that 0.22 g of C cm-3 should be used as a conversion factor.