Immune responses of human dental pulp stem cells in lipopolysaccharide-induced microenvironment
Immune responses of human dental pulp stem cells in lipopolysaccharide-induced microenvironment
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DOI：
10.1002/cbin.10938
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发表时间：
2018-07-01
影响因子：
3.9
通讯作者：
Chai, Wen Lin
中科院分区：
文献类型：
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作者：
Bindal, Priyadarshini;Ramasamy, Thamil Selvee;Chai, Wen Lin
本研究旨在探讨炎症刺激对牙髓干细胞（DPSCs）增殖的影响，以及脂多糖（LPS）诱导的牙髓干细胞微环境（iDPSCs）中基因和蛋白的表达。首先表征DPSC的间充质性质，然后用一系列LPS浓度从12至72小时对其进行攻击。在此之后，评估其基于增殖和炎症的基因以及蛋白质表达。iDPSC已经显示出间充质标志物的显著表达。与对照组相比，LPS暴露后，细胞活力随LPS浓度的增加而明显下降（P
This study aimed to investigate the effect of inflammatory stimuli on dental pulp stem cells (DPSCs) by assessing their proliferation and expression of genes as well as proteins in lipopolysaccharide (LPS)-induced microenvironment (iDPSCs). DPSCs were first characterized for their mesenchymal properties prior to challenging them with a series of LPS concentrations from 12 to 72h. Following to this, their proliferation and inflammatory based genes as well as protein expression were assessed. iDPSCs had demonstrated significant expression of mesenchymal markers. Upon exposure to LPS, the viability dropped distinctly with increasing concentration, as compared to control (P