One-pot enzymatic synthesis of the Gal alpha 1->3Cal beta 1->4GlcNAc sequence with in situ UDP-Gal regeneration
One-pot enzymatic synthesis of the Gal alpha 1->3Cal beta 1->4GlcNAc sequence with in situ UDP-Gal regeneration
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DOI：
10.1007/bf00731458
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发表时间：
1996-08-01
影响因子：
3
通讯作者：
VandenEijnden, DH
中科院分区：
文献类型：
--
作者：
Hokke, CH;Zervosen, A;VandenEijnden, DH
三糖Gal α 1->3Gal β 1->4GlcNAc β 1->O-（CH 2）（8）COOCH 3是酶促合成的，具有原位UDP-Gal再生。通过在一个罐中组合仅四种酶，即蔗糖合成酶、UDP-Glc 4 ′-差向异构酶、UDP-Gal：GlcNAc β 4-半乳糖基转移酶和UDP-Gal：Gal β 1->4GlcNAc α 3-半乳糖基转移酶，从受体GlcNAc β 1->O-（CH 2）（8）COOCH 3开始以2.2 μ mol ml（-1）产率形成Gal α 1->3Gal β 1->4GlcNAc β 1->O-（CH 2）（8）COOCH 3。这是合成Gal α 1->3Gal β 1->4GlcNAc表位的有效和方便的方法，所述表位在各种生物学和免疫学过程中起重要作用。
The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)(8)COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)(8)COOCH3 was formed in a 2.2 mu mol ml(-1) yield starting from the acceptor GlcNAc beta 1-->O-(CH2)(8)COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which plays an important role in various biological and immunological processes.