Antibodies to coronaviruses are higher in older compared with younger adults and binding antibodies are more sensitive than neutralizing antibodies in identifying coronavirus-associated illnesses
Antibodies to coronaviruses are higher in older compared with younger adults and binding antibodies are more sensitive than neutralizing antibodies in identifying coronavirus-associated illnesses
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DOI：
10.1002/jmv.25715
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发表时间：
2020-03-03
影响因子：
12.7
通讯作者：
Patel, Gira B.
中科院分区：
文献类型：
--
作者：
Gorse, Geoffrey J.;Donovan, Mary M.;Patel, Gira B.
人类冠状病毒（HCoV）是呼吸道疾病（RI）的常见原因，尽管预先存在体液免疫。作为2009年至2013年评价RI的200例受试者的前瞻性研究的一部分，在RI发作前后和3至4周后获得血清。通过酶联免疫吸附试验和中和试验比较患有心肺疾病的老年人（99例受试者）与年轻健康成人（101例受试者），测量针对常见HCoV菌株的抗体。通过聚合酶链反应检测呼吸道分泌物中的病毒脱落。在43例HCoV相关疾病中，15例（35%）发生在14例老年人（年龄≥ 60岁）中，28例（65%）发生在28例年轻人（年龄21-40岁）中。结合和中和抗体在老年人中较高。仅16例（35.7%）结合抗体增加的RI也增加了HCoV中和抗体。RI结合抗体的增加比中和抗体和病毒脱落的增加更常见，年轻人比老年人更常见。功能性中和抗体不像结合抗体那样经常受到刺激，部分解释了HCoV再感染的易感性。监测结合抗体可能对HCoV感染的血清学检测更敏感。
Human coronaviruses (HCoV) are common causes of respiratory illnesses (RI) despite preexisting humoral immunity. Sera were obtained near the onset of RI and 3 to 4 weeks later as part of a prospective study of 200 subjects evaluated for RI from 2009 to 2013. Antibodies against common HCoV strains were measured by enzyme-linked immunosorbent assay and neutralization assay comparing older adults with cardiopulmonary diseases (99 subjects) to younger, healthy adults (101 subjects). Virus shedding was detected in respiratory secretions by polymerase chain reaction. Of 43 HCoV-associated illnesses, 15 (35%) occurred in 14 older adults (aged >= 60 years) and 28 (65%) in 28 younger adults (aged 21-40 years). Binding and neutralizing antibodies were higher in older adults. Only 16 (35.7%) of RI with increases in binding antibodies also had increases in neutralizing antibodies to HCoV. Increases in binding antibodies with RI were more frequent than increased neutralizing antibodies and virus shedding, and more frequent in younger compared to older adults. Functional neutralizing antibodies were not stimulated as often as binding antibodies, explaining in part a susceptibility to reinfection with HCoV. Monitoring binding antibodies may be more sensitive for the serologic detection of HCoV infections.