PROTEIN REFOLDING USING AQUEOUS 2-PHASE SYSTEMS
PROTEIN REFOLDING USING AQUEOUS 2-PHASE SYSTEMS
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DOI：
10.1016/0021-9673(94)80096-0
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发表时间：
1994-05-06
影响因子：
4.1
通讯作者：
FORCINITI, D
中科院分区：
文献类型：
--
作者：
FORCINITI, D
我们提出了一种新的重折叠技术，其中水两相系统被用来解离蛋白质聚集体（包涵体）和重折叠的蛋白质在只有一个操作。三种变性剂盐（硫氰酸钠，氯化钙和溴化锂）被用作与聚（乙二醇）（PEG）的相形成盐。我们已经通过溶解碳酸酐酶II聚集体并在PEG-NaCl-NaSCN系统中重折叠变性蛋白质来测试该技术。
We present a novel refolding technique in which aqueous two-phase systems are used to both dissociate protein aggregates (inclusion bodies) and to refold the protein in only one operation. Three denaturant salts (sodium thiocyanate, calcium chloride, and lithium bromide) were used as phase-forming salts with poly(ethylene glycol) (PEG). We have tested the technique by dissolving carbonic anhydrase II aggregates and refolding the denatured protein in PEG-NaCl-NaSCN systems.