Lentiviral Vectors
Lentiviral Vectors
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DOI：
10.1007/978-1-61779-095-9_8
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发表时间：
2011-01-01
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影响因子：
--
通讯作者：
Salmon, Patrick
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文献类型：
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作者：
Giry-Laterriere, Marc;Verhoeyen, Els;Salmon, Patrick
在过去的十年中，慢病毒载体已经发展成为强大、可靠和安全的工具，用于在各种哺乳动物细胞中稳定地进行基因转移。与其他来源于逆转录病毒的载体相反，它们可以稳定地将基因传递到大多数未分裂的原代细胞中。特别是，来自HIV-1的慢载体（LVs）已经逐渐进化出许多理想的特征，旨在提高它们的安全性和多功能性。这就是为什么慢病毒载体正在成为基因工程最有用和最有前途的工具，以产生可用于研究、诊断和治疗的细胞。本章描述了lv的生产和滴定的协议和指南，可以在研究实验室环境中实施，重点是标准化，以提高实验室之间结果的可转换性。我们还讨论了低压技术的最新设计。
Lentiviral vectors have evolved over the last decade as powerful, reliable, and safe tools for stable gene transfer in a wide variety of mammalian cells. Contrary to other vectors derived from oncoretroviruses, they allow for stable gene delivery into most nondividing primary cells. In particular, lentivectors (LVs) derived from HIV-1 have gradually evolved to display many desirable features aimed at increasing both their safety and their versatility. This is why lentiviral vectors are becoming the most useful and promising tools for genetic engineering, to generate cells that can be used for research, diagnosis, and therapy.This chapter describes protocols and guidelines, for production and titration of LVs, which can be implemented in a research laboratory setting, with an emphasis on standardization in order to improve transposability of results between laboratories. We also discuss latest designs in LV technology.