Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum
Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum
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DOI：
10.1093/jxb/erg110
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发表时间：
2003-03-01
影响因子：
6.9
通讯作者：
Muthukrishnan, S
中科院分区：
文献类型：
--
作者：
Anand, A;Zhou, T;Muthukrishnan, S
利用基因枪转化技术，将从禾谷镰刀菌（Fusarium graminearum）侵染的抗赤霉病小麦品种“苏麦3号”（Sumai-3）穗部cDNA文库中分离的病程相关蛋白（PR-）基因转化到感病春小麦“Bobwhite”中，以期提高小麦对赤霉病的抗性水平。再生了24个表达单个PR蛋白基因或其组合的推定转基因株系。这些品系中的大多数（20）中的转基因表达在T-1或T-2代中完全沉默。四个转基因小麦品系表现出稳定的遗传和表达，无论是一个单一的转基因或转基因组合长达四代。当针对疮痂进行生物测定时，共表达几丁质酶和β-1，3-葡聚糖酶基因组合的一个系显示在温室条件下感染传播延迟（II型抗性）。在田间条件下，评价该品系和表达水稻奇异果甜蛋白样蛋白基因（tip）的第二转基因品系对赤霉病的抗性，所述转基因品系在先前的温室试验中对赤霉病具有中等抗性，沿着易感性和抗性检查。在田间强病原条件下，转基因株系对赤霉病无抗性，表明这些植株在这些条件下缺乏有效的抗初始侵染（I型抗性）。这是国内首次对转基因玉米进行田间评价。表达抗病PR蛋白基因的小麦。
Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance against scab. Twenty-four putative transgenic lines expressing either a single PR-protein gene or combinations thereof were regenerated. Transgene expression in a majority of these lines (20) was completely silenced in the T-1 or T-2 generations. Four transgenic wheat lines showed stable inheritance and expression of either a single transgene or transgene combinations up to four generations. One line co-expressing a chitinase and beta-1,3-glucanase gene combination, when bioassayed against scab showed a delay in the spread of the infection (type II resistance) under greenhouse conditions. This line and a second transgenic line expressing a rice thaumatin-like protein gene (tip) which had moderate resistance to scab in previous greenhouse trials, along with susceptible and resistance checks were evaluated for resistance to scab under field conditions. None of the transgenic lines had resistance to scab in the field under conditions of strong pathogen, suggesting these plants lacked effective resistance to initial infection (type I resistance) under these conditions. As far as is known, this is the first report of field evaluation of transgenic. wheat expressing genes for PR-proteins against disease resistance.