BACTEROIDES-VULGATUS OUTER-MEMBRANE ANTIGENS ASSOCIATED WITH CARRAGEENAN-INDUCED COLITIS IN GUINEA-PIGS
BACTEROIDES-VULGATUS OUTER-MEMBRANE ANTIGENS ASSOCIATED WITH CARRAGEENAN-INDUCED COLITIS IN GUINEA-PIGS
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DOI：
10.1128/iai.56.7.1754-1759.1988
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发表时间：
1988-07-01
影响因子：
3.1
通讯作者：
KASPER, DL
中科院分区：
文献类型：
--
作者：
BREELING, JL;ONDERDONK, AB;KASPER, DL
先前的溃疡性结肠炎卡拉胶模型实验表明，用普通拟杆菌（Bacteroides vulgatus）免疫豚鼠并随后给实验动物喂食这种细菌，可以增强豚鼠的炎症反应。本文报道的研究表明，EDTA从细菌外膜中提取的抗原是造成这种效果的原因。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对不同菌株的外膜蛋白及苯酚-水法提取的脂多糖进行了分析。虽然观察到的外膜蛋白模式很复杂，但根据对一组抗血清的免疫印迹，可以将菌株分为两种电泳类型（表型）。免疫印迹实验中使用的交叉吸收抗血清鉴定了四种与能够增强结肠炎炎症反应的现象独特相关的外膜蛋白。当在8%到12%的丙烯酰胺梯度十二烷基硫酸钠凝胶中测量时，这些蛋白质的分子量分别为100,000、57,000、34,000和21,000。鉴定出的其他抗原包括至少一种光滑的LPS，三种粗糙的LPS，以及在测试的普通芽孢杆菌菌株中一个30,000分子量的共同抗原。外膜制剂用于动物免疫和攻毒实验，结肠炎的严重程度与一种电泳类型相关。讨论了膜蛋白在结肠炎增强中的潜在作用。
Previous experiments with the carrageenan model for ulcerative colitis demonstrated that the inflammatory response in guinea pigs can be enhanced by immunization with Bacteroides vulgatus and subsequent feeding of this organism to experimental animals. The studies reported here show that antigens extractable from the bacterial outer membrane by EDTA are responsible for this effect. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the outer membrane proteins from various strains as well as the lipopolysaccharides (LPS) extractable by the phenol-water method. Although the observed pattern of outer membrane proteins was complex, the strains could be divided into two electrophoretic types (phenons) on the basis of immunoblotting against a panel of antisera. Cross-absorbed antisera used in immunoblotting experiments identified four outer membrane proteins uniquely associated with the phenon capable of enhancing the colitis inflammatory response. These proteins had molecular weights of 100,000, 57,000, 34,000, and 21,000 when measured in 8% to 12% acrylamide gradient sodium dodecyl sulfate gels. Other antigens identified included at least one type of smooth LPS, three types of rough LPS, and a common antigen of 30,000 molecular weight among the strains of B. vulgatus tested. The outer membrane preparations were used in animal immunization and challenge experiments, and the severity of colitis was correlated with one electrophoresis type. The potential role of membrane proteins in the enhancement of colitis is discussed.