The complete mitochondrial genome of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae:: gene order and trn gene clusters reveal a common evolutionary course for all Sordariomycetes, while intergenic regions show variation
The complete mitochondrial genome of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae:: gene order and trn gene clusters reveal a common evolutionary course for all Sordariomycetes, while intergenic regions show variation
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DOI：
10.1007/s00203-006-0104-x
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发表时间：
2006-06-01
影响因子：
2.8
通讯作者：
Typas, Milton A.
中科院分区：
文献类型：
--
作者：
Ghikas, Dimitri V.;Kouvelis, Vassili N.;Typas, Milton A.
昆虫病原真菌绿僵菌（Metarhizium anisopliae var. anisopliae）的线粒体基因组（mtDNA）总大小为24673 bp，是已知的绿僵菌属中最小的mtDNA之一。它包含14个编码氧化磷酸化相关蛋白的典型基因，2个rRNA基因，1个内含子，内含子ORF编码大rRNA基因（rnl）中的核糖体蛋白（rps），以及一组24个tRNA基因，这些基因识别除脯氨酸和缬氨酸外的所有氨基酸的密码子。与所有已知的sordariomyetes mtdna的基因顺序比较表明，所有蛋白质和rrna编码基因以及三个tRNA基因簇具有高度保守的基因组组织。通过将所有线粒体必需蛋白质编码基因作为一个单元，对这些小基因组的系统发育研究有力地支持了Sordariomycetes的共同进化过程（100% bootstrap支持），并突出了分析小基因组（mtDNA）相对于单基因的优势。此外，对三个基因间区域的比较分析表明，序列变异可用于绿僵菌种内和种间鉴定。
The mitochondrial genome (mtDNA) of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, with a total size of 24,673 bp, was one of the smallest known mtDNAs of Pezizomycotina. It contained the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, a single intron that harbored an intronic ORF coding for a putative ribosomal protein (rps) within the large rRNA gene (rnl), and a set of 24 tRNA genes which recognized codons for all amino acids, except proline and valine. Gene order comparison with all known mtDNAs of Sordariomycetes illustrated a highly conserved genome organization for all the protein- and rRNA-coding genes, as well as three clusters of tRNA genes. By considering all mitochondrial essential protein-coding genes as one unit a phylogenetic study of these small genomes strongly supported the common evolutionary course of Sordariomycetes (100% bootstrap support) and highlighted the advantages of analyzing small genomes (mtDNA) over single genes. In addition, comparative analysis of three intergenic regions demonstrated sequence variability that can be exploited for intra- and inter-specific identification of Metarhizium.