The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use
The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use
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DOI：
10.1111/j.1365-2591.2005.01028.x
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发表时间：
2005-11-01
影响因子：
5
通讯作者：
Ford, TRP
中科院分区：
文献类型：
--
作者：
Camilleri, J;Montesin, FE;Ford, TRP
目的通过细胞代谢功能和细胞增殖评价三氧化矿物骨料和加速波特兰水泥及其洗脱液的生物相容性。方法灰色和白色波特兰水泥的化学组成，灰色和白色矿物三氧化物骨料（MTA）和通过从生产过程中排除石膏而生产的加速波特兰水泥（奥尔堡白色）使用X射线能量色散分析和X射线衍射分析两者来确定。使用直接测试方法和间接测试方法评估材料的生物相容性，在直接测试方法中，使用Alcohol Blue（TM）染料定量测量细胞增殖，在间接测试方法中，细胞在材料洗脱液上生长，并使用国际标准指南推荐的甲基四唑测定法评估细胞增殖。ISO 10993-第5部分：体外试验结果所有受试材料的化学组成相似。间接研究表明，与对照组相比，洗脱液在培养24 h后的细胞活性增加（P < 0.05）。直接细胞接触的水泥导致细胞活力下降的所有时间点研究（P < 0.001）。结论骨水泥洗脱液的生物相容性测试表明，没有有毒的可降解物从灰色或白色MTA的存在下，氧化铋的加速波特兰水泥没有干扰生物相容性。新的加速波特兰水泥显示出类似的结果。当与试验骨水泥直接接触接种时，细胞生长较差。然而，由水合反应期间产生的氢氧化钙组成的洗脱液显示出诱导细胞增殖。
Aim To evaluate the biocompatibility of mineral trioxide aggregate and accelerated Portland cement and their eluants by assessing cell metabolic function and proliferation.Methodology The chemical constitution of grey and white Portland cement, grey and white mineral trioxide aggregate (MTA) and accelerated Portland cement produced by excluding gypsum from the manufacturing process (Aalborg White) was determined using both energy dispersive analysis with X-ray and X-ray diffraction analysis. Biocompatibility of the materials was assessed using a direct test method where cell proliferation was measured quantitatively using Alamar Blue(TM) dye and an indirect test method where cells were grown on material elutions and cell proliferation was assessed using methyltetrazolium assay as recommended by the International standard guidelines, ISO 10993-Part 5 for in vitro testing.Results The chemical constitution of all the materials tested was similar. Indirect studies of the eluants showed an increase in cell activity after 24 h compared with the control in culture medium (P < 0.05). Direct cell contact with the cements resulted in a fall in cell viability for all time points studied (P < 0.001).Conclusions Biocompatibility testing of the cement eluants showed the presence of no toxic leachables from the grey or white MTA, and that the addition of bismuth oxide to the accelerated Portland cement did not interfere with biocompatibility. The new accelerated Portland cement showed similar results. Cell growth was poor when seeded in direct contact with the test cements. However, the elution made up of calcium hydroxide produced during the hydration reaction was shown to induce cell proliferation.