The role of the methylene blue and toluidine blue monomers and dimers in the photoinactivation of bacteria
The role of the methylene blue and toluidine blue monomers and dimers in the photoinactivation of bacteria
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DOI：
10.1016/j.jphotobiol.2003.06.002
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发表时间：
2003-10-15
影响因子：
5.4
通讯作者：
Biel, MA
中科院分区：
文献类型：
--
作者：
Usacheva, MN;Teichert, MC;Biel, MA
用荧光光度法研究了吩噻嗪染料亚甲基蓝（MB）和甲苯胺蓝（TB）与细菌（金黄色葡萄球菌、肺炎链球菌、粪肠球菌、流感嗜血杆菌、大肠杆菌和铜绿假单胞菌）的相互作用。这表明染料和细菌之间发生了异染反应。此外，细菌诱导MB和TB的额外二聚化。在浓度为10（8）CFU/ml的每种细菌菌株存在下，评价MB和TB的有效二聚常数。在细菌存在下MB和TB的有效二聚常数的分析表明，TB形成二聚体的能力大于MB。革兰氏阴性菌比革兰氏阳性菌更强烈地诱导染料二聚化。每种染料在细菌存在下形成二聚体的能力与每种染料对这些细菌的相对光杀菌功效之间存在相关性。这些结果提供了证据，证实了染料二聚体在细菌光损伤中的重要作用。(C)2003 Elsevier B. V.保留所有权利。
The interactions between the phenothiazine dyes, methylene blue (MB) and toluidine blue (TB), and bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis, Hemophilus influenzae, Escherichia coli and Pseudomonas aeruginosa) were studied spectrophotometrically. This demonstrated that a metachromatic reaction took place between the dyes and bacteria. Furthermore, bacteria induced additional dimerization of MB and TB. The effective dimerization constants of MB and TB were evaluated in the presence of each bacterial strain at a concentration of 10(8) CFU/ml. The analysis of the effective dimerization constants for MB and TB in the presence of bacteria indicated that the ability to form dimers was greater for TB than for MB. Gram-negative bacteria induced the dye dimerization more intensely than gram-positive bacteria. There was a correlation between the ability of each dye to form dimers in the presence of bacteria and the relative photobactericidal efficacy of each dye against these bacteria. These results provide evidence confirming the essential role of the dye dimers in bacterial photodamage. (C) 2003 Elsevier B.V. All rights reserved.