Rice MAPK phosphatase IBR5 negatively regulates drought stress tolerance in transgenic Nicotiana tabacum
Rice MAPK phosphatase IBR5 negatively regulates drought stress tolerance in transgenic Nicotiana tabacum
复制标题
水稻 MAPK 磷酸酶 IBR5 负调控转基因烟草的干旱胁迫耐受性
DOI：
10.1016/j.plantsci.2012.02.005
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发表时间：
2012-06-01
期刊：
影响因子：
5.2
通讯作者：
Wang, Jinfa
中科院分区：
文献类型：
--
作者：
Li, Yuge;Feng, Dongru;Wang, Jinfa
丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）磷酸酶（mitogen-activated protein kinase phosphatases，MKPs）是MAPK信号通路中重要的负调控因子，在植物生长发育调控和环境胁迫应答中起着重要作用。一些MAPKs已被报道参与干旱胁迫反应，然而，没有证据表明MKPs在干旱胁迫中的特异性功能。在这里，一个假定的MKP在水稻（水稻），OsIBR 5，其特征在于。用PEG 6000、脱落酸（阿坝）和过氧化氢（H2 O2）诱导OsIBR 5的表达。OsIBR 5在烟草中的过表达导致了对干旱和H2 O2处理的敏感性。与对照相比，干旱和ABA诱导的气孔关闭在OsIBR 5过表达的烟草植物中显著降低。此外，OsIBR 5被发现与烟草MAPK SIPK和WIPK相互作用，并且在OsIBR 5过表达的烟草植株中干旱诱导的WIPK活性受损。这些结果表明，OsIBR 5是一种由非生物胁迫诱导的MKP，在转基因烟草中降低了对干旱胁迫的耐受性。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
The mitogen-activated protein kinase (MAPK) phosphatases (MKPs) are important negative regulators in the MAPK signaling pathways, which play crucial roles in plant growth, regulation of development and response to environment stresses. Several MAPKs have been reported to be involved in the drought stress response, however, there is no evidence for the specific function of MKPs in drought stress. Here, a putative MKP in rice (Oryza sativa), OsIBR5, was characterized. Expression of OsIBR5 was induced by PEG6000, abscisic acid (ABA) and hydrogen peroxide (H2O2). Overexpression of OsIBR5 in tobacco plants resulted in hypersensitivity to drought and H2O2 treatments. Drought and ABA-induced stomatal closure was significantly reduced in OsIBR5-overexpressing tobacco plants compared with controls. Moreover, OsIBR5 was found to interact with tobacco MAPKs SIPK and WIPK, and drought-induced WIPK activity was impaired in OsIBR5-overexpressing tobacco plants. These results indicated that OsIBR5 is a MKP which was induced by abiotic stresses and decreased tolerance to drought stress in transgenic tobacco plants. (C) 2012 Elsevier Ireland Ltd. All rights reserved.