Salvianolic Acid A, a Component of Salvia miltiorrhiza, Attenuates Endothelial-Mesenchymal Transition of HPAECs Induced by Hypoxia
Salvianolic Acid A, a Component of Salvia miltiorrhiza, Attenuates Endothelial-Mesenchymal Transition of HPAECs Induced by Hypoxia
复制标题
丹酚酸 A 是丹参的一种成分，可减弱缺氧诱导的 HPAEC 的内皮-间质转化
DOI：
10.1142/s0192415x17500653
复制
发表时间：
2017-01-01
影响因子：
5.7
通讯作者：
Du, Guanhua
中科院分区：
文献类型：
--
作者：
Yuan, Tianyi;Chen, Yucai;Du, Guanhua
Salvianolic acid A (SAA), a polyphenols acid, is a bioactive ingredient from a traditional Chinese medicine called Dan shen (Salvia Miltiorrhiza Bunge). According to previous studies, it was shown to have various effects such as anti-oxidative stress, antidiabetic complications and antipulmonary hypertension. This study aimed to investigate the effect of SAA on pulmonary arterial endothelial-mesenchymal transition (EndoMT) induced by hypoxia and the underlying mechanisms. Primary cultured human pulmonary arterial endothelial cells (HPAECs) were exposed to 1% O-2 for 48 h with or without SAA treatment. SAA treatment improved the morphology of HPAECs and inhibited the cytoskeleton remodeling. A total of 3 mu M SAA reduced migration distances from 262.2 mu m to 198.4 mu m at 24 h and 344.8 mu m to 109.3 mu m at 48 h. It was observed that the production of ROS in cells was significantly reduced by the treatment of 3 mu M SAA. Meanwhile, SAA alleviated the loss of CD31 and slightly inhibited the expression of alpha-SMA. The mechanisms study shows that SAA treatment increased the phosphorylation levels of Smad1/5, but inhibited that of Smad2/3. Furthermore, SAA attenuated the phosphorylation levels of ERK and Cofilin, which were enhanced by hypoxia. Based on these results, our study indicated that SAA treatment can protect HPAECs from endoMT induced by hypoxia, which may perform via the inhibition on ROS production and further through the downstream effectors of BMPRs or TGF beta R including Smads, ERK and ROCK/cofilin pathways.
