Complement receptor 3 (CD11b/CD18) is implicated in the elimination of β-amyloid peptides
Complement receptor 3 (CD11b/CD18) is implicated in the elimination of β-amyloid peptides
复制标题
DOI：
10.1111/j.1472-8206.2010.00811.x
复制
发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Gies, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Choucair-Jaafar, Nada;Laporte, Vincent;Gies, Jean-Pierre
小胶质细胞是大脑的专业吞噬细胞，表达补体受体3 （CR3或CD11b/CD18）等吞噬受体。利用热杀酵母制成的淀粉样沉积的模拟物，涂上A β 1-40或A β 1-42，我们能够在体外研究小胶质细胞如何与这些颗粒相互作用并摄入这些颗粒。我们之前已经证明，低密度脂蛋白受体相关蛋白（LRP）在很大程度上暗示了β 1-42-调理热死酵母的吞噬作用，部分暗示了β 1-40-调理热死酵母的吞噬作用。在这里，我们报道了针对CD11b或CD18的抗体减少了小胶质细胞对人工淀粉样蛋白沉积的摄取，表明CR3参与了这一机制。此外，LRP和CR3的同时抑制完全阻断了这两种颗粒的摄入，表明没有其他受体参与这一机制。
Microglia are the professional phagocytes of the brain and express phagocytic receptors such as complement receptor 3 (CR3 or CD11b/CD18). Using mimics of the amyloid deposit made of heat-killed yeasts coated with either A beta 1-40 or A beta 1-42, we were able to study how microglia interacted with and ingested these particles in vitro. We have shown previously that the low density lipoprotein receptor-related protein (LRP) is largely implied in the phagocytosis of A beta 1-42-opsonized heat-killed yeasts and partly in that of A beta 1-40-opsonized heat-killed yeasts. Here, we report that antibodies against CD11b or CD18 reduced the uptake of the artificial amyloid deposit by microglial cell showing that CR3 is involved in the mechanism. Moreover, a concomitant inhibition of LRP and CR3 completely blocked the ingestion of both kinds of particles suggesting that no other receptors participate to this mechanism.