SPECIFIC LYMPHOPROLIFERATION, GAMMA-INTERFERON PRODUCTION, AND SERUM IMMUNOGLOBULIN-G DIRECTED AGAINST A PURIFIED 32-KDA MYCOBACTERIAL PROTEIN ANTIGEN (P-32) IN PATIENTS WITH ACTIVE TUBERCULOSIS
SPECIFIC LYMPHOPROLIFERATION, GAMMA-INTERFERON PRODUCTION, AND SERUM IMMUNOGLOBULIN-G DIRECTED AGAINST A PURIFIED 32-KDA MYCOBACTERIAL PROTEIN ANTIGEN (P-32) IN PATIENTS WITH ACTIVE TUBERCULOSIS
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DOI：
10.1111/j.1365-3083.1988.tb02338.x
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发表时间：
1988-02-01
影响因子：
3.7
通讯作者：
DEBRUYN, J
中科院分区：
文献类型：
--
作者：
HUYGEN, K;VANVOOREN, JP;DEBRUYN, J
检测了21例活动性结核病患者对纯化的蛋白衍生物（PPD）和纯化的32-kDa蛋白抗原（P32）的免疫反应性。外周血白细胞对PPD和P32的增殖反应阳性率分别为95%和71%。阳性IFN-γ在62%的患者中检测到对PPD的应答，在48%的患者中检测到对P32的应答。低胚细胞生成和IFN-γ特别是在一般健康状况不佳和晚期结核病损的患者中。结核菌素阳性的健康志愿者中12人（100%）对PPD和P32有反应，平均淋巴细胞增殖和IFN-γ。高于患者组的数值。12例结核菌素阴性对照者对PPD和P32抗原完全无反应。结核菌素阳性或阴性的健康志愿者血清中均未检测到IgG抗体，PPD抗体阳性率为95%，P32抗体阳性率为77%。在具有最低体外淋巴细胞增殖和IFN-γ的那些患者中发现抗PPD的最高IgG水平。产奶量（r=-0.54; P < 0.05）。在对照组和患者组中，用纽卡斯尔病病毒、短小棒状杆菌或植物血凝素诱导后产生的非特异性干扰素相当。最后，低IFN-α。在约50%的患者的血清中检测到滴度。
Twenty-one patients treated for active tuberculosis were examined for immune reactivity to purified protien derivative (PPD) and to a purified 32-kDa protein antigen (P32) from Mycobacterium bovis, strain BCG. Lymphoproliferation of peripheral blood leucocytes to PPD and P32 was positive in 95% and 71% of the patients respectively. A positive IFN-.gamma. response was detected in 62% against PPD and in 48% against P32. Low blastogenesis and IFN-.gamma. production were observed, especially in patients with poor general health and advanced tuberculous lesions. Twelve out of twelve (100%) of the tuberculin-positive healthy volunteers responded to PPD and P32 with mean lymphoproliferation and IFN-.gamma. values that were higher than in the patient group. Twelve tuberculin-negative control subjects were completely unreactive to PPD and P32 antigen. On the other hand, IgG antibodies in the serum were detected in 95% of the patients against PPD, in 77% of the patients against P32 but in none of the tuberculin-positive or negative healthy volunteers. The highest IgG levels against PPD were found in those patients with the lowest in vitro lymphoproliferation and IFN-.gamma. production (r=-0.54; P < 0.05). Non-specific interferon production following induction with Newcastle disease virus, Corynebacterium parvum, or phytohaemagglutinin was comparable in the control and patient groups. Finally, low IFN-.alpha. titres were detected in the serum of about 50% of the patients.