Analysis of global host gene expression during the primary phase of the Arabidopsis thaliana-Plasmodiophora brassicae interaction
Analysis of global host gene expression during the primary phase of the Arabidopsis thaliana-Plasmodiophora brassicae interaction
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DOI：
10.1071/fp11026
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Cahill, David M.
中科院分区：
文献类型：
--
作者：
Agarwal, Arati;Kaul, Vijay;Cahill, David M.
利用基因芯片技术研究了感病植物拟南芥（Arabidopsis thaliana（L.）Heynh生态型Col-0和活体营养型病原菌芸苔根肿菌Woronin。在接种后4、7和10天（DAI）进行分析，结果显示在一系列功能类别中，相对少量的基因受到显著诱导或抑制。在4 DAI，有几个基因的诱导表达已知是关键的病原体识别和信号转导在其他耐药宿主-病原体相互作用。随着病原体进一步定殖根组织，并通过初级原质团阶段的进展，以生产游动孢子囊在7日和10日，分别，较少的基因表现出表达的变化。通过实时定量逆转录酶PCR（RT-qPCR）分析在4 DAI时检查诱导基因的子集来验证微阵列结果，所有这些都与微阵列数据呈正相关。两个A。发现测试的拟南芥突变体jar 1和coil 1对芸苔疫霉敏感。进一步研究了防御相关激素在相互作用中的作用，结果表明，添加水杨酸可以抑制A.拟南芥植物。
Microarray analysis was used to investigate changes in host gene expression during the primary stages of the interaction between the susceptible plant Arabidopsis thaliana (L.) Heynh ecotype Col-0 and the biotrophic pathogen Plasmodiophora brassicae Woronin. Analyses were conducted at 4, 7 and 10 days after inoculation (DAI) and revealed significant induction or suppression of a relatively low number of genes in a range of functional categories. At 4 DAI, there was induced expression of several genes known to be critical for pathogen recognition and signal transduction in other resistant host-pathogen interactions. As the pathogen further colonised root tissue and progressed through the primary plasmodium stage to production of zoosporangia at 7 and 10 DAI, respectively, fewer genes showed changes in expression. The microarray results were validated by examining a subset of induced genes at 4 DAI by quantitative real-time reverse transcriptase PCR (RT-qPCR) analysis all of which correlated positively with the microarray data. The two A. thaliana mutants jar1 and coiI tested were found to be susceptible to P. brassicae. The involvement of defence-related hormones in the interaction was further investigated and the findings indicate that addition of salicylic acid can suppress clubroot disease in A. thaliana plants.