Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs
Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs
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DOI：
10.1016/j.virusres.2012.04.014
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发表时间：
2012-08-01
期刊：
影响因子：
5
通讯作者：
Lu, Chengping
中科院分区：
文献类型：
--
作者：
Xu, Jiarong;Huang, Dongyan;Lu, Chengping
猪流感病毒（SIV）是猪的重要呼吸道病原，也是对人类健康的严重威胁。尽管用表达保护性抗原的重组牛痘病毒作为疫苗接种策略的免疫接种已广泛用于许多传染病，但用于此目的的重组猪痘病毒（rSPV）载体的开发不太成功。本文报道了表达H3 N2亚型SIV血凝素（HA 1）的重组猪痘病毒（rSPV-H3）的构建。在小鼠和猪模型中评估疫苗接种载体的免疫应答和保护效力。rSPV-H3的初免和加强接种产生了针对SIV的中和抗体，并从T淋巴细胞引发了有效的H3 N2 Sly特异性INF-γ应答。实现了猪对H3 N2 Sly攻击的完全保护。攻毒后未发现猪出现严重的全身和局部反应，也未发现从rSPV-H3免疫的猪身上脱落的SW。结果表明，H3 N2 Sly HA 1基因的重组表达载体具有良好的免疫保护作用。(c)2012 Elsevier B. V.保留所有权利。
Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rSPV) vector for this purpose has been less successful. Here, we report the construction of a recombinant swinepox virus (rSPV) expressing hemagglutinin (HA1) of H3N2 SIV (rSPV-H3). Immune responses and protection efficacy of the vaccination vector were assessed in both mouse and pig models. Prime and boost inoculations of rSPV-H3 yielded neutralization antibody against SIV and elicited potent H3N2 Sly-specific INF-gamma response from T-lymphocytes. Complete protection of pigs against H3N2 Sly challenge was achieved. No pigs showed severe systemic and local reactions and no SW was found shed from the pigs vaccinated with rSPV-H3 after challenge. The data suggest that the SPV-based recombinant vector expressing HA1 of H3N2 Sly might serve as a promising SIV vaccine for protection against SIV infection. (c) 2012 Elsevier B.V. All rights reserved.