Generation of multiple electrosprays using microfabricated emitter arrays for improved mass spectrometric sensitivity
Generation of multiple electrosprays using microfabricated emitter arrays for improved mass spectrometric sensitivity
复制标题
DOI：
10.1021/ac001191r
复制
发表时间：
2001-04-15
影响因子：
7.4
通讯作者：
Smith, RD
中科院分区：
文献类型：
--
作者：
Tang, KQ;Lin, YH;Smith, RD
采用激光刻蚀技术在聚碳酸酯基底上制备了微电喷雾发射器阵列。稳定的多电喷雾成功地产生在液体流量范围内相关的质谱应用，从微制造的发射器阵列和传统的熔融石英毛细管产生的电喷雾的比较显示出相似的喷雾特性和可靠性。在给定的总液体流速下，随着电喷雾次数的增加，观察到更高的总电喷雾离子电流。与理论预测一致，在恒定的总液体流速下的总喷雾电流被实验证明近似与电喷雾数量的平方根成比例。进一步预测，当总流率被优化时，最大可实现的总电流将与发射器的数量成比例。使用三重四极杆质谱仪的多电喷雾装置的评价显示，在相同的操作条件下，与传统的单电喷雾电离源相比，喷雾数范围从2到9的灵敏度提高了2-3倍。
Arrays of microelectrospray emitters were fabricated on polycarbonate, substrates using a laser etching technique. Stable multielectrosprays were successfully generated in the liquid flow rate range relevant to mass spectrometric applications, Comparison of electrosprays generated from the microfabricated emitter array and conventional fused-silica capillaries showed similar spray characteristics and reliability. Higher total electrospray ion currents were observed as the number of electrosprays increased at a given total liquid flow rate. Consistent with the theoretical prediction, the total spray current at a constant total liquid flow rate was shown experimentally to be approximately proportional to the square root of the number of electrosprays. It is further projected that when total flow rate is optimized the maximum achievable total current will be proportional to the number of emitters. Evaluation of the multielectrospray device using a triple quadrupole mass spectrometer showed a factor of 2-3 sensitivity enhancement for the spray numbers ranging from two to nine compared to a conventional single electrospray ionization source under the same operating conditions.