Inhibition of vancomycin-resistant enterococci by an in vitro continuous-flow competitive exclusion culture containing human stool flora
Inhibition of vancomycin-resistant enterococci by an in vitro continuous-flow competitive exclusion culture containing human stool flora
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DOI：
10.1086/324533
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发表时间：
2001-12-15
影响因子：
6.4
通讯作者：
Rice, LB
中科院分区：
文献类型：
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作者：
Donskey, CJ;Hume, ME;Rice, LB
采用体外厌氧连续流竞争排出(CFCE)培养模型，研究了人粪便菌群对万古霉素耐药肠球菌(VRE)的抑制作用。CFCE培养是从一名健康成年人的粪便样本中建立的。在CFCE培养液中加入10(3)或10(6)cfu/mLVR型粪肠球菌后，VRE在6天或9天内即可被清除。在含有10(7)cfu/mLVRE的连续流培养中加入10(7)cfu/mLCFCE时，VRE的密度降低，但不能消除。这些数据支持本土肠道菌群抑制VRE生长的假设，并表明CFCE培养可能是研究本土菌群与VRE之间相互作用的有用手段。
An in vitro anaerobic continuous-flow competitive exclusion (CFCE) culture model was used to study the ability of human stool flora to inhibit the growth of vancomycin-resistant (VR) enterococci (VRE). The CFCE culture was established from a stool sample obtained from a healthy adult. When 10(3) or 10(6) cfu/mL of VR Enterococcus faecium were added to the CFCE culture, the VRE were eliminated within 6 or 9 days, respectively. When 10(7) cfu/mL of the CFCE culture was added to a continuous-flow culture that contained 10(7) cfu/mL of VRE, the density of VRE was reduced but not eliminated. These data support the hypothesis that the indigenous intestinal flora inhibit growth of VRE and suggest that CFCE cultures may be a useful means to study interactions between the indigenous flora and VRE.