Laser-scanning velocimetry: a confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae.
Laser-scanning velocimetry: a confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae.
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DOI：
10.1186/1472-6750-7-40
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发表时间：
2007-07-10
影响因子：
3.5
通讯作者：
Johnson GL
中科院分区：
文献类型：
--
作者：
Malone MH;Sciaky N;Stalheim L;Hahn KM;Linney E;Johnson GL
斑马鱼斑马鱼是药物发现和研究心血管发育的重要模型系统。使用激光扫描共焦显微镜，我们开发了一种测量斑马鱼胚胎和幼虫心脏性能的非侵入性方法，该方法获得的心血管参数类似于在哺乳动物中使用多普勒超声心动图获得的参数。与背主动脉中的血液路径平行放置的激光扫描线测量血细胞速度，由此计算心输出量以及血管阻力和收缩力指数。这种技术被称为激光扫描测速，用于量化药理学、发育和遗传修饰剂对心脏功能的影响。应用激光扫描测速技术分析 MEKK3 (OSM) 渗透传感支架吗啉代敲低对心血管的影响，MEKK3 (OSM) 突变时会导致人类血管疾病脑海绵状血管瘤。 OSM缺陷的胚胎具有收缩的主动脉弓和显着增加的峰值细胞速度，这是主动脉瓣狭窄的特征指标。这些数据验证了激光扫描测速法作为测量斑马鱼心血管性能的定量工具，用于药理和遗传分析，除了激光扫描共聚焦显微镜外不需要任何专用设备。
The zebrafish Danio rerio is an important model system for drug discovery and to study cardiovascular development. Using a laser-scanning confocal microscope, we have developed a non-invasive method of measuring cardiac performance in zebrafish embryos and larvae that obtains cardiovascular parameters similar to those obtained using Doppler echocardiography in mammals. A laser scan line placed parallel to the path of blood in the dorsal aorta measures blood cell velocity, from which cardiac output and indices of vascular resistance and contractility are calculated. This technique, called laser-scanning velocimetry, was used to quantify the effects of pharmacological, developmental, and genetic modifiers of cardiac function. Laser-scanning velocimetry was applied to analyze the cardiovascular effects of morpholino knockdown of osmosensing scaffold for MEKK3 (OSM), which when mutated causes the human vascular disease cerebral cavernous malformations. OSM-deficient embryos had a constricted aortic arch and markedly increased peak cell velocity, a characteristic indicator of aortic stenosis. These data validate laser-scanning velocimetry as a quantitative tool to measure cardiovascular performance for pharmacological and genetic analysis in zebrafish, which requires no specialized equipment other than a laser-scanning confocal microscope.
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影响因子：
2.5
作者：
Liebling, Michael;Forouhar, Arian S.;Dickinson, Mary E.
通讯作者：
Dickinson, Mary E.
DOI：
10.1152/ajpheart.2000.278.4.h1142
发表时间：
2000-04-01
影响因子：
4.8
作者：
Osterloh, K;Gaehtgens, P;Pries, AR
通讯作者：
Pries, AR
DOI：
10.1098/rstb.2000.0629
发表时间：
2000-07-29
影响因子：
6.3
作者：
Warren, KS;Wu, JC;Fishman, RC
通讯作者：
Fishman, RC
影响因子：
3.5
作者：
Pan, Xiaotao;Yu, Hanry;Wohland, Thorsten
通讯作者：
Wohland, Thorsten
DOI：
10.1111/j.1540-8175.1990.tb00344.x
发表时间：
1990-01-01
期刊：
Echocardiography (Mount Kisco, N.Y.)
影响因子：
--
作者：
Saeian, K;Wann, L S;Sagar, K B
通讯作者：
Sagar, K B