Temporal variability in urinary excretion of bisphenol A and seven other phenols in spot, morning, and 24-h urine samples
Temporal variability in urinary excretion of bisphenol A and seven other phenols in spot, morning, and 24-h urine samples
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DOI：
10.1016/j.envres.2013.07.001
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发表时间：
2013-10-01
影响因子：
8.3
通讯作者：
Andersson, Anna-Maria
中科院分区：
文献类型：
--
作者：
Lassen, Tina Harmer;Frederiksen, Hanne;Andersson, Anna-Maria
在流行病学研究中很难确定人类接触现代非持久性化学品的情况，因为接触模式和排泄率可能会随时间和昼夜变化。本研究的目的是评估双酚A（BPA）和其他七种酚类的尿排泄重复测量的时间变异性。所有分析物测定使用TurboFlow-LC-MS/MS。两个点，三个第一个早晨和三个24小时的尿液样本收集了33个年轻的丹麦男子在三个月内。通过组内相关系数（ICC）估计时间变异性。超过70%的尿样中含有可检测水平的BPA、三氯生（TCS）、二苯甲酮-3（BP-3）以及2，4-二氯苯酚和2，5-二氯苯酚（Sigma DCP）。我们发现BPA（0.10-0.42）和Sigma DCP（0.39-0.72）的ICC为低至中度，而BP-3（0.69-0.80）和TCS（0.55-0.90）的ICC较高。与24小时尿样和第一次晨尿样品（间隔约40天采集）相比，两次现场尿样（间隔约4天采集）的ICC最高。BPA尿液排泄的显著差异可能导致流行病学研究中对个体BPA暴露水平的错误分类，这可能导致BPA与结局之间的相关性减弱。我们的数据不支持收集24小时样本将改善个人暴露评估的任何分析酚。(C)2013 Elsevier Inc. All rights reserved.
Human exposure to modern non-persistent chemicals is difficult to ascertain in epidemiological studies as exposure patterns and excretion rates may show temporal and diurnal variations. The aim of this study was to assess the temporal variability in repeated measurements of urinary excretion of bisphenol A (BPA) and seven other phenols. All analytes were determined using TurboFlow-LC-MS/MS. Two spot, three first morning and three 24-h urine samples were collected from 33 young Danish men over a three months period. Temporal variability was estimated by means of intraclass correlation coefficients (ICCs). More than 70% of the urine samples had detectable levels of BPA, triclosan (TCS), benzophenone-3 (BP-3) and sum of 2,4-dichlorophenol and 2,5-dichlorophenol (Sigma DCP). We found low to moderate ICCs for BPA (0.10-0.42) and Sigma DCP (0.39-0.72), whereas the ICCs for BP-3 (0.69-0.80) and TCS (0.55-0.90) were higher. The ICCs were highest for the two spot urine samples, which were collected approximately 4 days apart, compared with the 24-h urine samples and the first morning urine samples, which were collected approximately 40 days apart. A consequence of the considerable variability in urinary excretion of BPA may be misclassification of individual BPA exposure level in epidemiological studies, which may lead to attenuation of the association between BPA and outcomes. Our data do not support that collection of 24-h samples will improve individual exposure assessment for any of the analysed phenols. (C) 2013 Elsevier Inc. All rights reserved.