Multi-tissue integration of transcriptomic and specialized metabolite profiling provides tools for assessing the common bean (Phaseolus vulgaris) metabolome
Multi-tissue integration of transcriptomic and specialized metabolite profiling provides tools for assessing the common bean (Phaseolus vulgaris) metabolome
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DOI：
10.1111/tpj.14178
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发表时间：
2019-03-01
期刊：
影响因子：
7.2
通讯作者：
Fernie, Alisdair R.
中科院分区：
文献类型：
--
作者：
de Souza, Leonardo Perez;Scossa, Federico;Fernie, Alisdair R.
菜豆（Phaseolus vulgaris L.）是一种重要的豆科植物，具有丰富的地方品种的自然多样性，起源于野生形式，经过多次独立的驯化事件。在其基因组公布后，已经提供了这种相关作物的几种资源。然而，仍然缺乏对普通疟原虫专门代谢的全面表征。在这项研究中，我们使用了代谢组学方法的基础上，液相色谱-质谱法解剖的化学成分在组织特异性水平在几个加入普通菜豆属于不同的基因库。结合文献检索、质谱解释、C-13标记和相关性分析，我们能够为约39%的所有测量代谢物分配化学类别和/或推定结构。此外，我们将这些信息与转录组学数据和来自多个豆类物种的系统发育推断相结合，以重建可能的代谢途径，并确定参与专门代谢物生物合成的候选基因集。特别关注黄酮类化合物，三萜皂苷和羟基肉桂酸酯，因为它们代表参与重要生态相互作用的代谢物，并且当融入人类饮食时，它们也与几种促进健康的益处有关。这里的数据是以一种可访问的资源的形式呈现的，我们希望这将为进一步研究豆类的专门代谢奠定基础。
Common bean (Phaseolus vulgaris L.) is an important legume species with a rich natural diversity of landraces that originated from the wild forms following multiple independent domestication events. After the publication of its genome, several resources for this relevant crop have been made available. A comprehensive characterization of specialized metabolism in P. vulgaris, however, is still lacking. In this study, we used a metabolomics approach based on liquid chromatography-mass spectrometry to dissect the chemical composition at a tissue-specific level in several accessions of common bean belonging to different gene pools. Using a combination of literature search, mass spectral interpretation, C-13-labeling, and correlation analyses, we were able to assign chemical classes and/or putative structures for approximately 39% of all measured metabolites. Additionally, we integrated this information with transcriptomics data and phylogenetic inference from multiple legume species to reconstruct the possible metabolic pathways and identify sets of candidate genes involved in the biosynthesis of specialized metabolites. A particular focus was given to flavonoids, triterpenoid saponins and hydroxycinnamates, as they represent metabolites involved in important ecological interactions and they are also associated with several health-promoting benefits when integrated into the human diet. The data are presented here in the form of an accessible resource that we hope will set grounds for further studies on specialized metabolism in legumes.