Development of a Bead-Based Multiplex Immunoassay for Simultaneous Quantitative Detection of IgG Serum Antibodies against Measles, Mumps, Rubella, and Varicella-Zoster Virus
Development of a Bead-Based Multiplex Immunoassay for Simultaneous Quantitative Detection of IgG Serum Antibodies against Measles, Mumps, Rubella, and Varicella-Zoster Virus
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DOI：
10.1128/cvi.05537-11
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发表时间：
2012-03-01
影响因子：
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通讯作者：
Berbers, Guy A. M.
中科院分区：
文献类型：
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作者：
Smits, Gaby P.;van Gageldonk, Pieter G.;Berbers, Guy A. M.
酶联免疫吸附试验(ELISA)通常用于定量检测血清中抗麻疹、腮腺炎、风疹和水痘-带状疱疹病毒(MMRV)的抗体。然而，这种方法既耗时又耗材。为此，建立了同时定量检测MMRV抗体的多重免疫分析方法。既可以使用公司内部的抗原，也可以使用商业上可用的抗原，使所有实验室都可以使用这种检测方法。与单独的ELISA相比，多重检测具有更高的敏感性和高度的特异性，只需要5亩L血清。异源抑制率不超过11.5%，同源抑制率在91.3%~97.9%之间。与麻疹(R-2=0.98)、流行性腮腺炎(R-2=0.97)、风疹(R-2=0.97)病毒的ELISA和水痘-带状疱疹病毒的ELISA试剂盒具有良好的相关性(R-2=0.95)。总之，MMRV多重试验是传统ELISA的一种很好的替代方法，适用于血清监测和疫苗研究。
Enzyme-linked immunosorbent assay (ELISA) is normally used to quantify the amount of serum IgG antibodies against measles, mumps, rubella, and varicella-zoster virus (MMRV). However, this method is time-and material-consuming. Therefore, a multiplex immunoassay for the simultaneous quantitative detection of antibodies against MMRV was developed. In-house as well as commercially available antigens can be used, making the assay available for all laboratories. The multiplex assay is much more sensitive than the separate ELISAs and has a high specificity, and only 5 mu l of serum is needed. Heterologous inhibition did not exceed 11.5%, while homologous inhibition varied between 91.3 and 97.9%. Good correlations with the in-house ELISAs for measles (R-2 = 0.98), mumps (R-2 = 0.97), and rubella (R-2 = 0.97) virus as well as with the ELISA kit for varicella-zoster virus (R-2 = 0.95) were obtained. In conclusion, the MMRV multiplex assay is a good alternative to the conventional ELISAs and suitable for use in serosurveillance and vaccine studies.