Factors affecting purification of CD34(+) peripheral blood stem cells using the Baxter Isolex 300i.
Factors affecting purification of CD34(+) peripheral blood stem cells using the Baxter Isolex 300i.
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影响使用 Baxter Isolex 300i 纯化 CD34( ) 外周血干细胞的因素。
DOI：
10.1089/15258160260194875
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Raymond
中科院分区：
文献类型：
--
作者：
J. Gryn;R. Shadduck;J. Lister;Z. Zeigler;J. Raymond
共有201例乳腺癌、卵巢癌或血液恶性肿瘤患者接受了使用化疗和粒细胞集落刺激因子（G-CSF）的外周血干细胞（PBSC）动员。使用巴克斯特CS 3000分离机收集干细胞产物。使用巴克斯特Isolex 300 i对单采产品进行240次CD 34（+）细胞分离。分析影响CD 34（+）细胞产量和纯度的因素。总收率为55%，总纯度为91.7%。T细胞污染限于总细胞的0.43%。包括红细胞（RBC）浓度、血小板浓度、CD 34（+）细胞浓度、选择的总WBC和处理前时间在内的变量对产量和纯度几乎没有影响。在Isolex 300 i中安装2.5版软件显示出产率和纯度的适度提高。患者在接受大剂量化疗后，将冻存的CD 34（+）选择细胞回输。未观察到输注相关副作用。使用CD 34（+）选择性细胞进行的移植数据分析显示，当移植<5.0 × 10（6）CD 34（+）细胞/kg时，血小板移植延迟或失败的风险增加。巴克斯特Isolex 300 i在广泛的起始条件下提供了可重现的CD 34（+）细胞纯化。为了提供迅速的植入，每公斤应输注>5.0 x 10（6）CD 34（+）细胞用于移植。
A total of 201 patients with breast cancer, ovarian cancer, or hematological malignancies underwent mobilization of peripheral blood stem cells (PBSC) using chemotherapy and granulocyte-colony stimulating factor (G-CSF). Stem cell products were collected using the Baxter CS3000 pheresis machine. The Baxter Isolex 300i was used to perform 240 CD34(+) cell separations on the apheresis products. Factors affecting yield and purity of the CD34(+) cells were analyzed. Overall yield was 55% and overall purity was 91.7%. T cell contamination was limited to 0.43% of total cells. Variables including red blood cells (RBC) concentration, platelet concentration, CD34(+) cell concentration, total WBCs selected, and time until processing had little effect on yields and purities. Installation of version 2.5 of the software in the Isolex 300i showed a modest improvement in yield and purity. Patients were reinfused with the cryopreserved CD34(+) selected cells following high-dose chemotherapy. No infusion-related side effects were noted. Analysis of engraftment data using the CD34(+)-selected cells revealed an increased risk of delayed or failed platelet engraftment when <5.0 x 10(6) CD34(+) cells per kilogram were transplanted. The Baxter Isolex 300i provides reproducible CD34(+) cell purification over a wide range of starting conditions. To provide prompt engraftment, >5.0 x 10(6) CD34(+) cells per kilogram should be infused for transplantation.
影响因子：
20.3
作者：
Davis Jm;S. Rowley;H. Braine;S. Piantadosi;G. Santos
通讯作者：
Davis Jm;S. Rowley;H. Braine;S. Piantadosi;G. Santos