Differences in Retinal Structure and Function between Aging Male and Female Sprague-Dawley Rats are Strongly Influenced by the Estrus Cycle.
Differences in Retinal Structure and Function between Aging Male and Female Sprague-Dawley Rats are Strongly Influenced by the Estrus Cycle.
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DOI：
10.1371/journal.pone.0136056
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lachapelle P
中科院分区：
文献类型：
--
作者：
Chaychi S;Polosa A;Lachapelle P
生理性别和年龄被认为是可能影响视网膜功能和结构的两个重要因素，这种影响可能受性激素如雌激素的影响。本研究的目的是阐明生物学性别和年龄对啮齿动物视网膜功能和结构的影响，并阐明发情周期对雌性大鼠视网膜功能的影响。用视网膜电图（ERG）对50只正常雄性和雌性白化病Sprague-Dawley（SD）大鼠在生后30、60、100、200和300天（n = 5-6只雄性和雌性大鼠/日龄）的视网膜功能进行了观察。ERG记录后，对两种性别进行视网膜组织学检查。同时比较绝经前和绝经后P540雌性大鼠的视网膜功能。在我们的动物模型中观察到视网膜结构和功能的性别和年龄相关的变化。然而，无论年龄如何，在雄性和雌性大鼠的ERG和视网膜组织学中均未观察到显著差异。尽管如此，我们还是注意到，在P60和P200之间，雌性大鼠的ERG振幅与雄性大鼠相比逐渐增加。此外，与年龄匹配的绝经雌性大鼠以及雄性大鼠相比，18月龄绝经前雌性大鼠的ERG（P540）更大。结果表明，生物学性别和年龄对白化病SD大鼠视网膜功能和结构有影响。此外，我们发现，周期性雌性大鼠具有更好的视网膜功能相比，绝经期雌性大鼠表明，有利的影响，动情周期的视网膜功能。
Biological sex and age are considered as two important factors that may influence the function and structure of the retina, an effect that might be governed by sexual hormones such as estrogen. The purpose of this study was to delineate the influence that biological sex and age exert on the retinal function and structure of rodents and also clarify the effect that the estrus cycle might exert on the retinal function of female rats. The retinal function of 50 normal male and female albino Sprague-Dawley (SD) rats was investigated with the electroretinogram (ERG) at postnatal day (P) 30, 60, 100, 200, and 300 (n = 5–6 male and female rats/age). Following the ERG recording sessions, retinal histology was performed in both sexes. In parallel, the retinal function of premenopausal and menopausal female rats aged P540 were also compared. Sex and age-related changes in retinal structure and function were observed in our animal model. However, irrespective of age, no significant difference was observed in ERG and retinal histology obtained from male and female rats. Notwithstanding the above we did however notice that between P60 and P200 there was a gradual increase in ERG amplitudes of female rats compared to males. Furthermore, the ERG of premenopausal female rats aged 18 months old (P540) was larger compared to age-matched menopausal female rats as well as that of male rats. Our results showed that biological sex and age can influence the retinal function and structure of albino SD rats. Furthermore, we showed that cycled female rats have better retinal function compared to the menopausal female rats suggesting a beneficial effect of the estrus cycle on the retinal function.