Galactose-induced Aging Model in Rat Testicular Tissue
Galactose-induced Aging Model in Rat Testicular Tissue
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DOI：
10.29271/jcpsp.2018.07.501
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发表时间：
2018-07-01
影响因子：
1
通讯作者：
Cakatay, Ufuk
中科院分区：
文献类型：
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作者：
Aydin, Seval;Yanar, Karolin;Cakatay, Ufuk
目的：检验D-半乳糖诱导的衰老模型是否是进一步衰老研究的合适模型。研究设计：实验研究。研究地点和持续时间：土耳其伊斯坦布尔大学Aziz Sancar实验医学研究所，2015年6月至2017年6月。方法：该研究包括3组大鼠。 I 组是 5 个月大的年轻对照 (YC) 大鼠。 II组是5个月大的大鼠，通过腹腔内给予D-半乳糖（60 mg/kg体重/天，0.5 mL）进行模拟老化（MA）6周。第 III 组是自然衰老 (NA) 24 个月大的大鼠。 I 组和 III 组接受腹腔内盐水 (0.9% 0.5 mL) 作为媒介物，为期 6 周。 I组和II组大鼠在21周龄时接受注射，III组大鼠在24月龄前6周接受注射。当大鼠6.5个月大（第一组和第二组）和24个月大（第三组）时收获组织。对蛋白质、脂质、DNA 生物标志物和 Cu、Zn-SOD 进行定量生化分析。使用方差分析对数据进行统计分析，然后进行事后 Bonferroni 检验。结果：在模拟老化和自然老化的睾丸组织中都发现了更严重的氧化损伤和减弱的抗氧化防御能力。结果发现，D-半乳糖衰老模型组在氧化还原稳态受损方面与自然衰老大鼠有显着相似性。结论：D-半乳糖诱导的睾丸衰老模型成功地模拟了衰老过程。因此，D-半乳糖诱导衰老模型可作为加速衰老模型来研究年龄相关的改变和干预。
Objective: To examine whether the D-galactose induced aging model is an appropriate model for further aging research.Study Design: Experimental study.Place and Duration of Study: Aziz Sancar Institute of Experimental Medicine, Istanbul University, Turkey, June 2015-June 2017.Methodology: The study comprises 3 groups of rats. Group I is young control (YC) 5-month-old rats. Group II is 5-monthold rats, which were mimetically aged (MA) for 6 weeks via intraperitoneal D-galactose (60 mg/kg body weight/day, 0.5 mL) administration. Group III is naturally aged (NA) 24-month-old rats. Group I and III received intraperitoneal saline (0.9% 0.5 mL) for 6 weeks as vehicle. Group I and Group II received injections at 21 weeks age and Group III rats 6 weeks before 24 months age. Tissues were harvested when rats became 6.5-month-old (Group I and Group II) and 24-month-old (Group III). Quantitative biochemical analyses of proteins, lipids, DNA biomarkers and Cu, Zn-SOD were conducted. Statistical analysis of the data was conducted using ANOVA, followed by post-hoc Bonferroni test.Results: Higher magnitude of oxidative damage and diminished antioxidant defence capacity were found in both mimetically aged and naturally aged testicular tissues. It is observed that D-galactose aging model group shares significant similarities in terms of impaired redox homeostasis with the naturally aged rats.Conclusion: D-galactose induced testicular aging model successfully mimics aging process. Therefore, D-galactose induced aging model may be used as an accelerated aging model to study the age related alterations and interventions.