Ignicoccus gen. nov., a novel genus of hyperthermophilic, chemolithoautotrophic Archaea, represented by two new species, Ignicoccus islandicus sp nov and Ignicoccus pacificus sp nov.
Ignicoccus gen. nov., a novel genus of hyperthermophilic, chemolithoautotrophic Archaea, represented by two new species, Ignicoccus islandicus sp nov and Ignicoccus pacificus sp nov.
复制标题
DOI：
10.1099/00207713-50-6-2093
复制
发表时间：
2000-11-01
影响因子：
2.8
通讯作者：
Stetter, KO
中科院分区：
文献类型：
--
作者：
Huber, H;Burggraf, S;Stetter, KO
从大西洋（冰岛北部的Kolbeinsey海岭）和太平洋（北纬9度，西经104度）的海底热液系统中分离出了两种新的化能自养、产硫微生物。球状细胞在70-98 ℃的温度范围内生长（最佳温度约为90 ℃）。它们通过使用分子氢作为电子供体还原元素硫来获得能量。基于16 S rDNA的序列比较显示，这些生物体是古菌属的泉古菌分支的成员。他们代表了一个新的，深刻的分支谱系内的家庭脱硫球菌科。在DNA-DNA杂交实验中，这两种菌株表现出低水平的杂交，彼此和进一步代表这个家庭。因此，它们代表了一个新属，建议将其命名为新球菌属。目前该菌有两个新种，一个是冰岛嗜热球菌（模式菌株为Kol 8（T）= DSM 13165（T）= ATCC 700957（T）），另一个是太平洋嗜热球菌（模式菌株为LPC 33（T）= DSM 13166（T）= ATCC 700958（T））。
Two species of novel, chemolithoautotrophic, sulfidogenic micro-organisms were isolated from submarine hydrothermal systems in the Atlantic (at the Kolbeinsey Ridge north of Iceland) and in the pacific (at 9 degreesN, 104 degreesW). The coccoid cells grew within a temperature range of 70-98 degreesC (optimum around 90 degreesC). They gained energy by reduction of elemental sulfur using molecular hydrogen as the electron donor. 16S rDNA-based sequence comparisons revealed that the organisms are members of the crenarchaeal branch of the Archaea. They represent a new, deeply branching lineage within the family of the Desulfurococcaceae. In DNA-DNA hybridization experiments both strains exhibited low levels of hybridization to each other and to further representatives of this family. Therefore, they represent a new genus, for which the name Ignicoccus gen. nov. is proposed. At present it consists of two new species, Ignicoccus islandicus sp. nov. (type strain is Kol8(T) = DSM 13165(T) = ATCC 700957(T)) and Ignicoccus pacificus sp. nov. (type strain is LPC33(T) = DSM 13166(T) = ATCC 700958(T)).