Endothelin-1 is an autocrine factor in rat inner medullary collecting ducts.
Endothelin-1 is an autocrine factor in rat inner medullary collecting ducts.
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Endothelin-1 是大鼠内髓集合管的自分泌因子。
DOI：
10.1152/ajprenal.1992.263.4.f607
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Padilla,E
中科院分区：
文献类型：
--
作者：
Kohan,DE;Padilla,E
内皮素-1（ET-1）可能是内髓集合管（IMCD）生理调节的重要因子。这部分肾单位合成ET-1，表达内皮素受体，并通过降低Na（+）-K（+）-ATP酶活性和水转运对外源性ET-1作出反应。总之，这些发现表明ET-1在IMCD功能调节中的自分泌作用;然而，由于IMCD的极化性质，尚不清楚ET-1分泌、受体和受体活化是否发生在细胞的同一侧。为了研究这个问题，大鼠IMCD细胞在半透膜上生长至汇合。这些细胞呈现极性形态，具有高跨上皮电阻。免疫反应性ET-1主要分泌到基底外侧。此外，125 I-ET-1主要结合于基底外侧表面。最后，ET-1（10（-8）M）刺激前列腺素E2的生产只有当加入到基底侧。因此，这些数据表明，ET-1能够自分泌调节IMCD细胞，这种作用主要发生在基底外侧。
Endothelin-1 (ET-1) may be an important factor in the regulation of inner medullary collecting duct (IMCD) physiology. This segment of the nephron synthesizes ET-1, expresses endothelin receptors, and responds to exogenous ET-1 by reducing Na(+)-K(+)-ATPase activity and water transport. Taken together, these findings suggest an autocrine role for ET-1 in the regulation of IMCD function; however, because of the polarized nature of the IMCD, it is not known if ET-1 secretion, receptors, and receptor activation occur on the same side of the cell. To examine this question, rat IMCD cells were grown to confluence on semipermeable membranes. These cells exhibited polar morphology with high transepithelial electrical resistances. Immunoreactive ET-1 was secreted primarily into the basolateral side. Furthermore, 125I-ET-1 bound predominantly to the basolateral surface. Finally, ET-1 (10(-8) M) stimulated prostaglandin E2 production only when added to the basolateral side. These data indicate, therefore, that ET-1 is capable of autocrine regulation of IMCD cells and that this effect occurs predominantly on the basolateral side.