Photo and pH Stable, Highly-Luminescent Silicon Nanospheres and Their Bioconjugates for Immunofluorescent Cell Imaging
Photo and pH Stable, Highly-Luminescent Silicon Nanospheres and Their Bioconjugates for Immunofluorescent Cell Imaging
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DOI：
10.1021/ja808827g
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发表时间：
2009-04-01
影响因子：
15
通讯作者：
Lee, Shuit-Tong
中科院分区：
文献类型：
--
作者：
He, Yao;Su, Yuanyuan;Lee, Shuit-Tong
我们报道了一种新型的氧化硅纳米球(O-SiNSs)，它同时具有优异的水分散性、高的发光量子产率(PLQY)、超光稳定性、宽的pH稳定性和良好的生物相容性。值得注意的是，O-SiNSs的PLQY高达25%，在强紫外光照射下和pH范围为2-12的酸性到碱性环境中都是稳定的。据我们所知，这是第一个水分散硅纳米颗粒的例子，具有高的PLQY和强大的pH稳定性，适合于广泛的生物应用。此外，O-SiNSs很容易与抗体偶联，所得到的O-SiNSs/抗体生物偶联物成功地应用于免疫荧光细胞成像。结果表明，高发光和稳定的O-SiNSs/抗体生物偶联物是一种具有广泛生物应用前景的荧光探针，如长期和实时细胞标记。
We report a novel kind of oxidized silicon nanospheres (O-SiNSs), which simultaneously possess excellent aqueous dispersibility, high photoluminescent quantum yield (PLQY), ultra photostability, wide pH stability, and favorable biocompatibility. Significantly, the PLQY of the O-SiNSs is as high as 25%, and is stable under intense UV irradiation and in acidic-to-basic environments covering the pH range 2-12. To our best knowledge, it is the first example of water-dispersed silicon nanoparticles which possess both high PLQY and robust pH stability suitable for broad utility in bioapplications. Furthermore, the O-SiNSs are readily conjugated with antibody, and the resultant O-SiNSs/antibody bioconjugates are successfully applied in immunofluorescent cell imaging. The results show that the highly luminescent and stable O-SiNSs/antibody bioconjugates are promising fluorescent probes for wide-ranging bioapplications, such as long-term and real-time cellular labeling.