Autophagy induced by resveratrol suppresses α-MSH-induced melanogenesis
Autophagy induced by resveratrol suppresses α-MSH-induced melanogenesis
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DOI：
10.1111/exd.12337
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发表时间：
2014-03-01
影响因子：
3.6
通讯作者：
Cho, Dong-Hyung
中科院分区：
文献类型：
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作者：
Kim, Eun Sung;Chang, Huikyoung;Cho, Dong-Hyung
自噬通过自噬体和溶酶体的协同作用降解细胞成分和细胞器。虽然已知自噬主要调节细胞成分的周转，但自噬在黑色素形成中的作用尚未得到很好的解决。在这里，我们发现抑制自噬抑制白藜芦醇（RSV）的抗黑色素生成活性，白藜芦醇是一种众所周知的抗黑色素生成剂。RSV病毒强烈增加黑素细胞的自噬。然而，ATG5的缺失显著抑制rsv介导的抗黑素生成以及rsv诱导的黑素细胞自噬。此外，在α - msh处理的细胞中，抑制ATG5可恢复rsv介导的酪氨酸酶和TRP1的下调。最重要的是，电镜分析显示，在α - msh和RSV联合治疗后，自噬体吞噬了黑色素或黑素小体。综上所述，这些结果表明rsv介导的自噬调节黑色素生成。
Autophagy degrades cellular components and organelles through a cooperative process involving autophagosomes and lysosomes. Although autophagy is known to mainly regulate the turnover of cellular components, the role of autophagy in melanogenesis has not been well addressed. Here, we show that inhibition of autophagy suppresses the antimelanogenesis activity of resveratrol (RSV), a well-known antimelanogenic agent. RSV strongly increased autophagy in melanocytes. However, the depletion of ATG5 significantly suppressed RSV-mediated antimelanogenesis as well as RSV-induced autophagy in melanocytes. Moreover, suppression of ATG5 retrieved the RSV-mediated downregulation of tyrosinase and TRP1 in alpha-MSH-treated cells. Most importantly, electron microscopy analysis revealed that autophagosomes engulfed melanin or melanosomes after combined treatment of alpha-MSH and RSV. Taken together, these results suggest that RSV-mediated autophagy regulates melanogenesis.