Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9
Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9
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DOI：
10.18388/abp.2007_3289
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Yu, Long
中科院分区：
文献类型：
--
作者：
Liu, Shen;Huang, Chaoqun;Yu, Long
我们在这里报告的克隆和鉴定的一个新的人类短链淀粉酶/还原酶基因SCDR 9，分离自人类肝脏cDNA文库，并通过浏览UCSC基因组数据库映射到4q22.1。SCDR 9含有一个900 bp的开放阅读框，编码一个含有信号肽序列和adh_short结构域的蛋白。GFP定位显示SCDR 9蛋白集中在细胞质的某些位点，但不在ER中。在18种组织中的表达模式显示，SCDR 9在肝脏中高度表达。利用pET 28 A（+）表达载体从大肠杆菌中成功纯化出可溶性重组蛋白。本研究为进一步研究SCDR 9基因及其产物的功能提供了重要信息。
We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.