Initial characterization of monoclonal antibodies against human monocytes.
Initial characterization of monoclonal antibodies against human monocytes.
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抗人单核细胞单克隆抗体的初步表征。
DOI：
10.1073/pnas.77.11.6764
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发表时间：
1980
影响因子：
11.1
通讯作者：
Capra,JD
中科院分区：
文献类型：
--
作者：
Ugolini,V;Nunez,G;Smith,RG;Stastny,P;Capra,JD
用体细胞融合技术制备了三种抗人单核细胞的单克隆抗体。广泛的特异性分析表明，这些抗体与大多数（如果不是全部）人外周血单核细胞反应，而不与高度纯化的T或B细胞反应。这些抗体中的两个识别的单核细胞抗原的初步化学表征。该分子是表观分子量为200，000的单链多肽。这些试剂应证明在单核细胞分化障碍的临床定义，在单核细胞功能的研究，并在单核细胞表面抗原的遗传学和结构的阐明有用。
Three monoclonal antibodies against human monocytes have been produced by somatic cell fusion. Extensive specificity analysis suggests that these antibodies react with most if not all human peripheral blood monocytes and not with highly purified T or B cells. Initial chemical characterization of the monocyte antigen recognized by two of these antibodies is presented. The molecule is a single polypeptide chain with an apparent molecular weight of 200,000. These reagents should prove useful in the clinical definition of disorders of monocyte differentiation, in studies of monocyte function, and in the elucidation of the genetics and structure of monocyte cell surface antigens.