IDENTIFICATION OF ANDROGEN RECEPTORS IN NORMAL HUMAN OSTEOBLAST-LIKE CELLS
IDENTIFICATION OF ANDROGEN RECEPTORS IN NORMAL HUMAN OSTEOBLAST-LIKE CELLS
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DOI：
10.1073/pnas.86.3.854
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
SPELSBERG, TC
中科院分区：
文献类型：
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作者：
COLVARD, DS;ERIKSEN, EF;SPELSBERG, TC
性类固醇，雄激素和雌激素，是骨代谢的主要调节剂。然而，这些激素是否通过直接或间接机制作用于骨细胞仍不清楚。最近用于证明骨中雌激素受体的核结合测定[Eriksen，E. F.、科尔瓦德湾美国，贝格，N. J.，格雷厄姆，M。L.，Mann，K. G.，Spelsberg，T. C.和Riggs，B. L.（1988）Science 241，84-86]用于鉴定氚化合成雄激素[3 H]R1881（methyltrienolone）在25种人成骨细胞样细胞株中的21种（84%）中的特异性核结合，并且结合的类固醇受体的浓度为821 ± 1000。140（平均值±）SEM）分子/细胞核。结合是饱和的和类固醇特异性的。RNA印迹分析证实成骨细胞中雄激素受体基因表达。雄激素和雌激素受体的相对浓度进行了比较，通过测量特定的核雌激素结合。在30个细胞株中的27个（90%）中观察到[3 H]雌二醇的核结合;结合的雌二醇受体的浓度为1537 . ±.每个细胞核有221个分子。雄性和雌性的[3 H]R1881和[3 H]雌二醇的核结合位点浓度相似。我们的结论是雄激素和雌激素直接作用于人骨细胞通过各自的受体介导的机制。
The sex steroids, androgens and estrogens, are major regulators of bone metabolism. However, whether these hormones act on bone cells through direct or indirect mechanisms has remained unclear. A nuclear binding assay recently used to demonstrate estrogen receptors in bone [Eriksen, E. F., Colvard, D. S., Berg, N. J., Graham, M. L., Mann, K. G., Spelsberg, T. C. and Riggs, B. L. (1988) Science 241, 84-86] was used to identify specific nuclear binding of a tritiated synthetic androgen, [3H]R1881 (methyltrienolone), in 21 of 25 (84%) human osteoblast-like cell strains and a concentration of bound steroid receptors of 821 .+-. 140 (mean .+-. SEM) molecules per cell nucleus. Binding was saturable and steroid-specific. Androgen receptor gene expression in osteoblasts was confirmed by RNA blot analysis. Relative concentrations of androgen and estrogen receptors were compared by measuring specific nuclear estrogen binding. Nuclear binding of [3H]estradiol was observed in 27 of 30 (90%) cell strains; the concentration of bound estradiol receptor was 1537 .+-. 221 molecules per cell nucleus. The concentrations of nuclear binding sites were similar in males and females for both [3H]R1881 and [3H]estradiol. We conclude that both androgens and estrogens act directly on human bone cells through their respective receptor-mediated mechanisms.