Kinetic analysis shows that vitamin A disposal rate in humans is positively correlated with vitamin A stores
Kinetic analysis shows that vitamin A disposal rate in humans is positively correlated with vitamin A stores
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DOI：
10.1093/jn/138.5.971
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发表时间：
2008-05-01
影响因子：
4.2
通讯作者：
Green, Michael H.
中科院分区：
文献类型：
--
作者：
Cifelli, Christopher J.;Green, Joanne B.;Green, Michael H.
采用基于模型的房室分析法测定了营养良好的中国和美国成年人的维生素A（VA）动力学、储存和处置率。[H-2（8）]醋酸视黄酯（8.9 μ mol）口服给美国（n = 12; 59 ± 9岁; mean ± SD）和中国（n= 14，54 ± 4岁）成人，测定3 h至56 d血清示踪剂和VA浓度。使用Windows版本的模拟、分析和建模软件，我们确定美国受试者从给药到血清中出现标记视黄醇的平均时间（40.6 +/- 8.47 h）大于中国受试者（32.2 +/- 5.84 h; P < 0.01）。模型预测的总示踪质量（898 +/- 637 vs. 237 +/- 109 μ mol），处置率（14.7 +/- 5.87 vs. 5.58 +/- 2.04 μ mol/d），和系统停留时间（58.9 ± 28.7 vs.42.9 ± 14.6 d）在美国受试者中大于中国受试者（P < 0.05）。模型预测的VA质量和通过氘代视黄醇稀释估计的VA质量在3和24 d没有差异。VA处置率与中国（R-2 = 0.556）、美国（R-2 = 0.579）和所有受试者（R-2 = 0.808）的VA示踪质量呈正相关。此外，所有受试者的VA处置率与血清视黄醇池大小（R-2 = 0.227）和视黄醇浓度（R-2 = 0.330）显著相关。我们的研究结果支持这一假设，即VA商店的VA处置率在健康，营养良好的成年人的主要决定因素。
Vitamin A (VA) kinetics, storage, and disposal rate were determined in well-nourished Chinese and U.S. adults using model-based compartmental analysis. [H-2(8)]Retinyl acetate (8.9 mu mol) was orally administered to U.S. (n = 12; 59 +/- 9 y; mean +/- SD) and Chineseadults (n= 14, 54 +/- 4y) and serum tracer and VA concentrations were measured from 3 h to 56 d. Using the Windows version of the Simulation, Analysis and Modeling software, we determined that the average time from dosing until appearance of labeled retinol in serum was greater in U.S. subjects (40.6 +/- 8.47 h) than in Chinese subjects (32.2 +/- 5.84 h; P < 0.01). Model-predicted total traced mass (898 +/- 637 vs. 237 +/- 109 mu mol), disposal rate (14.7 +/- 5.87 vs. 5.58 +/- 2.04 mu mol/d), and system residence time (58.9 +/- 28.7 vs. 42.9 +/- 14.6 d) were greater in U.S. than in Chinese subjects (P < 0.05). The model-predicted VA mass and VA mass estimated by deuterated retinol dilution at 3 and 24 d did not differ. VA disposal rate was positively correlated with VA traced mass in Chinese (R-2 = 0.556), U.S. (R-2 = 0.579), and all subjects (R-2 = 0.808). Additionally, VA disposal rate was significantly correlated with serum retinol pool size (R-2 = 0.227) and retinol concentration (R-2 = 0.330) in all subjects. Our results support the hypothesis that VA stores are the principle determinant of VA disposal rate in healthy, well-nourished adults.