Evaluation of Replication and Cross-Reactive Antibody Responses of H2 Subtype Influenza Viruses in Mice and Ferrets
Evaluation of Replication and Cross-Reactive Antibody Responses of H2 Subtype Influenza Viruses in Mice and Ferrets
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DOI：
10.1128/jvi.00511-10
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发表时间：
2010-08-01
影响因子：
5.4
通讯作者：
Subbarao, Kanta
中科院分区：
文献类型：
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作者：
Chen, Grace L.;Lamirande, Elaine W.;Subbarao, Kanta
自 1968 年以来，H2 流感病毒就不再在人类中传播，因此，如果 H2 流感病毒重新出现，很大一部分人群可能会受到感染。因此，开发 H2 大流行性流感病毒候选疫苗应被视为大流行性流感防备规划的优先事项。我们选择了一组地理和时间上不同的野生型 H2 流感病毒，评估了复制动力学，并比较了这些病毒在小鼠和雪貂中诱导广泛交叉反应性抗体反应的能力。在小鼠和雪貂中，通过血凝抑制和微量中和测定测定，A/Japan/305/1957 (H2N2)、A/mallard/NY/1978 (H2N2) 和 A/swine/MO/2006 (H2N3) 均引发针对异源 H2 流感病毒的最广泛的交叉反应性抗体应答。这些数据表明，这三种病毒可能是开发 H2 大流行性流感减毒活病毒疫苗的合适候选者。
H2 influenza viruses have not circulated in humans since 1968, and therefore a large segment of the population would likely be susceptible to infection should H2 influenza viruses reemerge. The development of an H2 pandemic influenza virus vaccine candidate should therefore be considered a priority in pandemic influenza preparedness planning. We selected a group of geographically and temporally diverse wild-type H2 influenza viruses and evaluated the kinetics of replication and compared the ability of these viruses to induce a broadly cross-reactive antibody response in mice and ferrets. In both mice and ferrets, A/Japan/305/1957 (H2N2), A/mallard/NY/1978 (H2N2), and A/swine/MO/2006 (H2N3) elicited the broadest cross-reactive antibody responses against heterologous H2 influenza viruses as measured by hemagglutination inhibition and microneutralization assays. These data suggested that these three viruses may be suitable candidates for development as live attenuated H2 pandemic influenza virus vaccines.