Functional and spatial segregation of secretory vesicle pools according to vesicle age
Functional and spatial segregation of secretory vesicle pools according to vesicle age
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DOI：
10.1038/nature01389
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发表时间：
2003-03-13
期刊：
影响因子：
64.8
通讯作者：
Chow, RH
中科院分区：
文献类型：
--
作者：
Duncan, RR;Greaves, J;Chow, RH
突触终末和神经内分泌细胞充满分泌囊泡，只有少数囊泡停靠在质膜上并易于释放。其余的被认为构成了一个大的细胞质储备池，等待招募到易释放池（RRP）中进行胞吐。囊泡在募集过程中是如何被优先考虑的仍然是未知的：选择可能是随机的，或者最古老或最新的囊泡可能会受到青睐。在这里，我们使用随时间改变颜色的荧光货物蛋白（2）（3）显示，牛肾上腺嗜铬细胞中的囊泡根据年龄分离成不同的群体。新组装的囊泡在生物发生后不久在质膜上是不移动的（形态学上停靠）的，而较老的囊泡是移动的并且位于细胞的更深处。不同的促分泌素选择性地从RRP释放囊泡，或者令人惊讶地，选择性地从更深的细胞质池释放囊泡。因此，远非平等，囊泡根据年龄在功能和空间上分离。
Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable. The remainder are thought to constitute a large cytoplasmic reserve pool awaiting recruitment into the readily releasable pool (RRP) for exocytosis'. How vesicles are prioritized in recruitment is still unknown: the choice could be random, or else the oldest or the newest ones might be favoured. Here we show, using a fluorescent cargo protein(2) that changes colour with time(3), that vesicles in bovine adrenal chromaffin cells segregate into distinct populations, based on age. Newly assembled vesicles are immobile (morphologically docked) at the plasma membrane shortly after biogenesis, whereas older vesicles are mobile and located deeper in the cell. Different secretagogues selectively release vesicles from the RRP or, surprisingly, selectively from the deeper cytoplasmic pool. Thus, far from being equal, vesicles are segregated functionally and spatially according to age.