Detoxification of woody hydrolyzates with activated carbon for bioconversion to ethanol by the thermophilic anaerobic bacterium Thermoanaerobacterium saccharolyticum
Detoxification of woody hydrolyzates with activated carbon for bioconversion to ethanol by the thermophilic anaerobic bacterium Thermoanaerobacterium saccharolyticum
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DOI：
10.1016/j.biombioe.2010.10.021
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发表时间：
2011-01-01
影响因子：
6
通讯作者：
Kenealy, William R.
中科院分区：
文献类型：
--
作者：
Lee, Jung Myoung;Venditti, Richard A.;Kenealy, William R.
自水解是一种简单的、绿色的从生物质中回收糖的方法，仅使用热水。一个潜在的缺点是在自动水解过程中形成副产物，其可能干扰随后的水解和发酵成乙醇。在目前的工作中，自水解预水解混合硬木片脱毒与活性炭和副产物的去除效率以及糖的损失确定。用嗜热厌氧细菌对所得脱毒预水解物进行发酵以产生乙醇的评价。在预水解产物上2.5wt%水平的活性炭能够去除42%的甲酸、14%的乙酸、96%的羟甲基糠醛（HMF）和93%的糠醛。然而，8.9%的糖也被去除。HMF和糠醛的去除遵循预期的吸附等温线，但甲酸、乙酸和糖不遵循。用活性炭脱毒的混合硬木的自水解预水解产物可以用解糖热厌氧杆菌菌株MO 1442发酵，产率基本上为100%。T.解糖菌菌株M0 1442能够代谢水解产物中的葡萄糖、木糖和阿拉伯糖。结果表明，活性炭脱毒工艺通过去除有毒化合物（主要是HMF和糠醛）提高了乙醇产率，同时适度损失了可发酵糖。爱思唯尔有限公司出版
Autohydrolysis is a simple, green method of recovering sugars from biomass, using only hot water. One potential drawback is that byproducts are formed during the autohydrolysis process that could interfere with subsequent hydrolysis and fermentation to ethanol. In the present work, autohydrolysis prehydrolyzate from mixed hardwood chips was detoxified with activated carbon and the removal efficiency of byproducts as well as the loss of sugars determined. The resulting detoxified prehydrolyzate was evaluated for the fermentation to ethanol with a thermophilic anaerobic bacterium. Activated carbon at a 2.5 wt% level on the prehydrolyzate was able to remove 42% of formic acid, 14% of acetic acid, 96% of hydroxymethylfurfural (HMF) and 93% of the furfural. However, 8.9% of sugars were also removed. The removal of HMF and furfural follow expected adsorption isotherms but formic acid, acetic acid, and sugars did not. Autohydrolysis prehydrolyzates from mixed hardwood detoxified with activated carbon can be fermented with Thermoanaerobacterium saccharolyticum strain MO1442 with an essentially 100% yield. T. saccharolyticum strain MO1442 is able to metabolize the glucose, xylose, and arabinose in the hydrolyzate. The results showed the detoxification process with activated carbon improved the ethanol yields by the removal of toxic compounds, mainly HMF and furfural, with moderate loss of fermentable sugars. Published by Elsevier Ltd.